J Vet Sci.  2013 Sep;14(3):337-343. 10.4142/jvs.2013.14.3.337.

Evaluation of bone healing in canine tibial defects filled with cortical autograft, commercial-DBM, calf fetal DBM, omentum and omentum-calf fetal DBM

Affiliations
  • 1Department of Veterinary Surgery and Radiology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran. dr.bigham@gmail.com
  • 2Department of Veterinary Pathobiology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
  • 3Department of Veterinary Pathobiology, School of Veterinary Medicine, Shiraz University, Shiraz, Iran.

Abstract

The present study was conducted to compare the effects of xenogenic bovine fetal demineralized bone matrix (DBM), commercial DBM, omentum, omentum-calf fetal DBM, cortical autograft and xenogenic cartilage powder on the healing of tibial defects in a dog model to determine the best material for bone healing. Seven male adult mongrel dogs, weighing 26.2 +/- 2.5 kg, were used in this study. Seven holes with a diameter of 4-mm were created and then filled with several biomaterials. Radiographs were taken postoperatively on day 1 and weeks 2, 4, 6, 8. The operated tibias were removed on the 56th postoperative day and histopathologically evaluated. On postoperative days 14, 42 and 56, the lesions of the control group were significantly inferior to those in the other group (p < 0.05). On the 28th postoperative day, the autograft group was significantly superior to the control and omentum groups (p < 0.05). Moreover, calf fetal DBM was significantly superior to the control group. There was no significant difference between the histopathological sections of all groups. Overall, the omentum and omentum-DBM groups were superior to the control group, but inferior to the autograft, commercial-DBM, calf fetal DBM and calf fetal cartilage groups.

Keyword

autograft; bone healing; calf fetal-demineralized bone matrix; commercial demineralized bone matrix; omentum

MeSH Terms

Animals
Autografts/*transplantation
Biocompatible Materials/*therapeutic use
*Bone Regeneration
Cattle
Dogs
Male
Omentum/*transplantation
*Wound Healing
Biocompatible Materials

Figure

  • Fig. 1 Seven bone defects were created for implantation of seven different biomaterials in tibial bone.

  • Fig. 2 Radiological evaluation on the 14th (A), 28th (B), 42nd (C) and 58th (D) postoperative days. 1: control, 2: autograft, 3: omentum, 4: omentum-calf fetal demineralized bone matrix (DBM), 5: commercial DBM, 6: calf fetal-DBM, 7: cartilage group.

  • Fig. 3 Histopathological findings showed normal structure of trabecular bone in the defect area in all groups with various phases of remodeling. (A) Control. (B) Autograft. (C) Commercial-DBM. (D) Calf fetal-DBM. (E) Omentum. (F) Omentum-calf fetal DBM. (G) Cartilage group (H&E stain, ×10).


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