Korean J Gastroenterol.  1998 Oct;32(4):427-434.

Relation of Apoytosis and Cell Proliferation in Helicobactor pylori Infection

• Park IS
• Lee SI
• Lee KS
• Chung SS
• Chung BC
• Pack HJ

Abstract

BACKGROUND AND AIMS: Many studies have shown that Helicobacter pylori (H. pylori) infection is associated with gastroduodenal diseases. The pathogenic effects of H. pylori are associated with cytokine production, cell cycle progression, cellular proliferation and apoptosis. We performed this study to investigate the effect of H. pylori eradication on gastric mucosal cell apoptosis and proliferation.
METHODS
Gastroduodenoscopy was performed for 42 cases. Twenty two of them showed H. pylori-positive gastritis and the other 20 cases showed H. pylori-negative normal mucosa. H. pylori infection was assessed by rapid urease test and histology of biopsy specimens from gastric antrum. Ki-67 immunostaining of gastric biopsy specimens was used to measure mucosal cell proliferation in H. pylori positive gastritis before and after therapy. DNA frapnentation with terminal deoxynu- cleotidyl transferase-mediated deoxyuridine triphosphate nick-end labeling method was used for a marker of apoptosis.
RESULTS
Apoptosis was increased in gastric mucosal epithelium of patients with H. pylori-positive gastritis (p<0.05). H. pylori eradication therapy reduced apoptosis in antral mucosa (p<0.05) and the cell proliferation levels (p=0.051).
CONCLUSIONS
Induction of apoptosis in gastric epithelium by chronic stimulation of H. pylori could be involved in the pathogenesis of gastroduodenal diseases.

Keyword

Helicobacter pylori; Apoptosis; Cell proliferation; Ki-67

MeSH Terms

Biopsy