J Asthma Allergy Clin Immunol.  2003 Mar;23(1):53-62.

Comparison of Pharmacia CAP system and Auro Dex(R) Visual ENS(TM) screening test for detecting specific IgE in atopic patients

Affiliations
  • 1Department of Internal Medicine, Ewha Woman's University College of Medicine, Seoul, Korea. yjcho@ewha.ac.kr

Abstract

BACKGROUND AND OBJECTIVE
Auro Dex(R) Visual ENS(TM) allergy screening test is a simplified and newly developed method for the detection of allergen-specific IgE in human serum. This system has advantages in several ways compared to the Pharmacia CAP system, such as the need for relatively small amounts of serum, no expensive equipment and rapid detection. The purpose of this study is to evaluate the efficiency of Auro Dex(R) Visual ENS(TM) screening test for the detection of specific IgE compared to the Pharmacia CAP system in atopic patients. METHOD: In 27 atopic patients (M:F = 11:16, age:13-51 years, average 27.9+/-10.2 years) who had positive response on skin prick test, the Pharmacia CAP system for the sensitized allergen and Auro Dex(R) Visual ENS(TM) screening test were performed. For comparison, 5 normal subjects who had negative response on skin prick test were tested for 5 allergens(Dermatophagoides(D) farinae, D. pteronyssinus, cockroach, dog epithelium, cat epithelium) by the Pharmacia CAP system and Auro Dex(R) Visual ENS(TM) screening test. RESULTS: Using skin prick test results as the reference standards, the sensitivity of the Pharmacia CAP system and Auro Dex(R) Visual ENS(TM) screening test was 87.5%, 57.1%, respectively. The specificity of Pharmacia CAP system and Auro Dex(R) Visual ENS(TM) screening test were 100%. There was a significant correlation between the Auro Dex(R) Visual ENS(TM) and CAP system (D.f. r=0.755, D.p. r=0.856) for D. farinae and D.pteronyssinus. CONCLUSION: Auro Dex(R) Visual ENS(TM) screening test showed high specificity for detection of allergen-specific IgE and good correlation with the Pharmacia CAP system. This system may be useful in general practice. However, due to relatively low sensitivity to some antigens compared to skin prick test, further development may be necessary.

Keyword

Atopy; specific IgE; skin prick test; Pharmacia CAP system; Auro.Dex(r).Visual.ENS(TM) screening test

MeSH Terms

Animals
Cats
Cockroaches
Dogs
Epithelium
General Practice
Humans
Hypersensitivity
Immunoglobulin E*
Mass Screening*
Sensitivity and Specificity
Skin
Immunoglobulin E
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