Korean J Physiol Pharmacol.  2002 Apr;6(2):93-100.

Increase of Intracellular Ca2+ Concentration Induced by Lysophosphatidylcholine in Murine Aortic Endothelial Cells

• Zhu MH
• Park SJ
• Kim HJ
• Yang DK
• Suh SH
• So I
• Kim KW

Abstract

Effects of oxidized low-density lipoprotein (ox-LDL), l-alpha-stearoyl-lysophosphatidylcholine (LPC), on intracellular Ca2+ concentration were examined in mouse endothelial cells by measuring intracellular Ca2+ concentration ([Ca2+]i) with fura 2-AM and reverse transcription-polymerase chain reaction (RT-PCR). LPC increased [Ca2+]i under the condition of 1.5 mM [Ca2+]o but did not show any effect under the nominally Ca2+-free condition. Even after the store depletion with 30microM 2,5-di-tert- butylhydroquinone (BHQ) or 30microM ATP, LPC could still increase the [Ca2+]i under the condition of 1.5 mM [Ca2+]o. The time required to increase [Ca2+]i (about 1 minute) was longer than that for ATP-induced [Ca2+]i increase (10-30 seconds). LPC-induced [Ca2+]i increase was completely blocked by 1microM La3+. Transient receptor potential channel(trpc) 4 mRNA was detected with RT-PCR. From these results, we suggest that LPC increased [Ca2+]i via the increase of Ca2+ influx through the Ca2+ routes which exist in the plasma membrane.

Keyword

Oxidized low-density lipoprotein (ox-LDL); Lysophosphatidylcholine (LPC); 2.5-di-tert- butylhydroquinone (BHQ); Endothelial cell (EC)

MeSH Terms

Adenosine Triphosphate
Animals
Cell Membrane
Endothelial Cells*
Lipoproteins
Lysophosphatidylcholines*
Mice
RNA, Messenger
Adenosine Triphosphate
Lipoproteins
Lysophosphatidylcholines
RNA, Messenger