Mycobiology.  2002 Sep;30(3):146-153. 10.4489/MYCO.2002.30.3.146.

Multiple Symbiotic Associations Found in the Roots of Botrychium ternatum

• Lee JK
• Eom AH
• Lee SS

• KMID: 1612357
• DOI: http://doi.org/10.4489/MYCO.2002.30.3.146

Abstract

Two types of mycorrhizae, orchid (OM) and arbuscular mycorrhizae (AM), were observed in the cortical cells of Botrychium ternatum roots. The vesicles or arbuscules of AM fungi were examined and the fresh or digestive pelotons by other species of basidiomycetes were also observed in the roots under light microscope. These symbioses were, as the genomic DNAs extracted from roots of B. ternatum reacted with the specific primers, confirmed with PCR technique, being added to more strong evidences. These discoveries were rarely happened in the roots, especially a fern in nature. OM was observed in the roots of B. ternatum collected from the nationwide areas, whereas AM was only in the roots of B. ternatum collected from Chung-Buk areas. It is speculated that OM are associated with the nitrogen cycle in Islands and the growth of B. ternatum in the inland of Central Korea is related to both the phosphate and nitrogen cycle in the nature. The results suggest that B. ternatum is a typical species with two types of mycorrhizae under various growing conditions.

Keyword

Arbuscular mycorrhizae; Botrychium ternatum; Fern mycorrhiza; Mycorrhiza; Orchid mycorrhiza; PCR; Specific primer; Symbiosis

MeSH Terms

Basidiomycota
DNA
Ferns
Fungi
Islands
Korea
Mycorrhizae
Nitrogen Cycle
Polymerase Chain Reaction
Symbiosis
DNA