Korean J Physiol Pharmacol.  2005 Jun;9(3):137-141.

Effects of a delta-opioid Agonist on the Brainstem Vestibular Nuclear Neuronal Activity of Rats

• Kim TS
• Huang M
• Jang MJ
• Jeong HS
• Park JS

Abstract

This study was undertaken to investigate the effects of [D-Ala2, D-Leu5]-enkephalin (DADLE) on the spontaneous activity of medial vestibular nuclear neurons of the rat. Sprague-Dawley rats, aged 14 to 16 days, were anesthetized with ether and decapitated. After enzymatic digestion, the brain stem portion of medial vestibular nuclear neuron was obtained by micropunching. The dissociated neurons were transferred to a recording chamber mounted on an inverted microscope, and spontaneous action potentials were recorded by standard patch-clamp techniques. The spontaneous action potentials were increased by DADLE in 12 cells and decreased in 3 cells. The spike frequency and resting membrane potential of these cells were increased by DADLE. The depth of afterhyperpolarization was not affected by DADLE. The potassium currents were decreased in 20 cells and increased in 5 cells. These results suggest that DADLE increases the neuronal activity of the medial vestibular nuclear neurons by altering resting membrane potential.

Keyword

Medial vestibular nuclear neurons; Action potential; [D-Ala2, D-Leu5]-enkephalin (DADLE)

MeSH Terms

Action Potentials
Animals
Brain Stem*
Digestion
Enkephalin, Leucine-2-Alanine
Ether
Membrane Potentials
Neurons*
Patch-Clamp Techniques
Potassium
Rats*
Rats, Sprague-Dawley
Enkephalin, Leucine-2-Alanine
Ether
Potassium