Postnatal Development of Calbindin D-28k Immunoreactivities in the Canine Hippocampus

Yoon, Sang Pil; Chung, Yoon Young; Chang, In Youb; Kim, Jong Joong; Moon, Jeong Seok; Kim, Hong Soon

Abstract

The developmental expression of calcium-binding protein, calbindin D-28k (CB), during the first 6 months was studied in the canine hippocampus by immunohistochemistry. CB immunoreactivity appeared from on P0 in the dentate granule cells, mossy fibers in CA 3 area, CA 2 and CA 1 pyramidal cells, and interneurons in all regions. According to their morphology and location, these could represent the presumptive pyramidal cells and interneurons. From on P7, the CB immunoreactive pyramidal cells clearly distinguished and started to form two rows in CA 1 area as time progressed, while scattered multipolar neurons gradually decreased. CB immunoreactive cell processes increased in length up to P28. The adult-like distribution of CB immunoreactivity was established about P60. After P60, CB immunoreactivity appeared in dentate granule cells, mossy fibers in CA 3 area and pyramidal cells in CA 1 where formed two rows and CA 2 areas as well as in interneurons of the strata oriens and pyramidale. Taken together, developmental expression of CB in the canine hippocampus was summarized that CB imunoreactivity was observed in all regions on P0 and reached adult-like distribution about P60. These data also suggested the possibility of prenatal expression of CB on the basis of the staining pattern at P0.