Korean J Anat.  2002 Apr;35(2):113-123.

Postnatal Development of Parvalbumin and Calbindin D-28k Immunoreactivities in the Canine Anterior Cingulate Cortex: Transient Expression in Layer V Pyramidal Cells

• Yoon SP
• Ahn GH
• Moon JS
• Kim JJ
• You HJ
• Kim JH
• Chang IY

Abstract

We have examined the ontogeny of parvalbumin and calbindin D-28k immunoreactivities in the canine anterior cingulate cortex from the day of birth (P0) through P180. At P7, parvalbumin immunoreactivity appears firstly in layer VI multipolar cells. The parvalbumin immunoreactivity in GABAergic interneurons appears to follow an 'inside-out' gradient of radial mergence and reaches an adult-like pattern by the end of the 6th postnatal month. Immunoreactivity is limited mainly to developing nonpyramidal cells, whereas pyramid-like parvalbumin immunoreactive cells are transiently observed in layer V from the P14 to the P90. The developmental pattern of calbindin D-28k immunoreactivity differs from that of parvalbumin immunoreactivity. Calbindin D-28k immunoreactivity develops firstly in layer V pyramidal cells from P0, which continues through the third postnatal month. Calbindin D-28k immunoreactive interneurons are located in the infragranular layers and white matter at P0 and increase in both the supragranular and infragranular layers by P14. This is followed by an adult-like pattern at the P180. These data suggested that parvalbumin and calbindin D-28k may play a role in protecting immature neurons from intracelluar calcium influx during postnatal development.

Keyword

Parvalbumin; Calbindin D-28k; Anterior cingulate cortex; Postnatal development; Immunohistochemistry; Dog

MeSH Terms

Animals
Calbindins*
Calcium
Dogs
Gyrus Cinguli*
Immunohistochemistry
Interneurons
Neurons
Parturition
Pyramidal Cells*
Calcium