Korean J Physiol Pharmacol.  1998 Apr;2(2):217-223.

Electrolysis of physiological salt solution generates a factor that relaxes vascular smooth muscle

• Song PO
• Chang KC

Abstract

Oxygen-derived free radicals have been implicated in many important functions in the biological system. Electrical field stimulation (EFS) causes arterial relaxation in animal models. We found that EFS applied to neither muscle nor nerve but to Krebs solution caused a relaxation of rat aorta that had been contracted with phenylephrine. In the present study, therefore, we investigated the characteristics of this EIRF (electrolysis-induced relaxing factor) using rat isolated aorta.
Results
indicated that EIRF acts irrespective of the presence of endothelium. EIRF shows positive Griess reaction and is diffusible and quite stable. EIRF-induced relaxation was stronger on PE-contracted aorta than on KCl-contracted one, and inhibited by the pretreatment with methylene blue. Zaprinast, a cGMP-specific phosphodiesterase inhibitor, potentiated the EIRF-induced relaxation. NG-nitro-L-arginine, NO synthase inhibitor, did not inhibit the EIRF-induced relaxation. Deferroxamine, but not ascorbic acid, DMSO potentiated the EIRF-induced relaxation. These results indicate that electrolysis of Krebs solution produces a factor that relaxes vascular smooth muscle via cGMP-mediated mechanism.

Keyword

Vascular relaxation; cGMP; Electrolysis; Nitric oxygen; Oxygen radicals

MeSH Terms

Animals
Aorta
Ascorbic Acid
Dimethyl Sulfoxide
Electrolysis*
Endothelium
Free Radicals
Methylene Blue
Models, Animal
Muscle, Smooth, Vascular*
Nitric Oxide Synthase
Nitroarginine
Phenylephrine
Rats
Reactive Oxygen Species
Relaxation
Ascorbic Acid
Dimethyl Sulfoxide
Free Radicals
Methylene Blue
Nitric Oxide Synthase
Nitroarginine
Phenylephrine
Reactive Oxygen Species