Korean J Clin Microbiol.  2000 Mar;3(1):53-56.

Screening of Urine Culture Specimens by Gram Stain, Urinalysis and Urine Microscopic Examinations

• Chang CH
• Park TH
• Jeong YS
• Kim HH
• Hwang WJ

Abstract

BACKGROUND
The purpose of this study was to discover ways to screen urine culture specimens through Gram stains, urine stick analyses and microscopic examinations for the laboratory cost saving.
METHODS
One hundred and fifty-eight urine specimens for culture were included. Fifty uL of urine were inoculated onto one well each of 10-well slide, dried on the hot plate, and Gram-stained. The results combined with routine urinalyses including urine nitrite and leukocyte esterase, and pyuria, were compared with the routine culture results.
RESULTS
The screening of bacteriuria by Gram stains, urinalyses and microscopic examinations revealed the high sensitivity (91.9%) and negative predictive value(95.5%) with cost saving of 41.8% of inoculating media. Not considering the Gram stains, the screening revealed 83.8% sensitivity and 92.5% negative predictive value, even if the cost saving of inoculating media were as high as 50.1%.
CONCLUSION
It was demonstrated that it was sensitive and economic and produced rapid preliminary results to screen bacteriuria by the Gram stains combined with urinalyses and microscopic examinations.

Keyword

Gram stain; Urine; 10-well slide galss; Screening; Bacteriuria

MeSH Terms

Bacteriuria
Coloring Agents
Cost Savings
Leukocytes
Mass Screening*
Pyuria
Urinalysis*
Coloring Agents