Korean J Pathol.  2007 Feb;41(1):15-20.

Immunohistochemical Profile of Acute Cellular Rejection in Renal Allograft

• Park J
• Byun SW
• Yu E
• Park SK
• Han DJ
• Cho YM

Abstract

BACKGROUND: We wanted to find an adjunctive marker(s) in renal allograft biopsies for predicting acute cellular rejection (ACR), and so the expression patterns of immune-related molecules were compared between ACR, borderline ACR and non-ACR cases.
METHODS
The expression patterns of Fas ligand (FasL), HLA-DR, granzyme B, caspase-3, CD56, interferon stimulated growth factor-3 (ISGF-3), and CD53 were assessed via immunohistochemical study in 75 allograft biopsies from patients with ACR (n=19), borderline ACR (n=22), and non-ACR (n=34).
RESULTS
Compared to those of the non-ACR group, the ACR group revealed an elevated number of FasL positive interstitial inflammatory cells, HLA-DR positive tubular inflammatory cells, cytoplasmic caspase-3 positive tubular epithelial cells, granzyme B positive interstitial mononuclear inflammatory cells and CD53 positive interstitial inflammatory cells. The expression patterns of the borderline ACR group were similar to those of non-ACR group, except for the intensity of FasL in the tubular epithelial cells.
CONCLUSIONS
Immunohistochemical investigations of the adjunctive markers FasL, HLA-DR, granzyme B, caspase-3 and CD56 can be useful for making the diagnosis of ACR.

Keyword

Renal transplantation; Graft rejection; Cellular immunity; Immunohistochemistry

MeSH Terms

Allografts*
Biopsy
Caspase 3
Cytoplasm
Diagnosis
Epithelial Cells
Fas Ligand Protein
Graft Rejection
Granzymes
HLA-DR Antigens
Humans
Immunity, Cellular
Immunohistochemistry
Interferons
Kidney Transplantation
Caspase 3
Fas Ligand Protein
Granzymes
HLA-DR Antigens
Interferons