Korean J Phys Anthropol.
2007 Dec;20(4):257-265.
Comparison between Morphological Sex and Genotype Sex of Uzbekistan Ancient Bones Using Improved Amelogenin PCR Amplication Method
- Affiliations
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- 1Department of Science & Culture Graduate School, Chung-Ang University, Korea. skull@cau.ac.kr
- 2Department of Microbiology, College of Medicine, Chung-Ang University, Korea.
- 3Department of Life science, College of Natural Science, Chung-Ang University, Korea.
- 4Department of Anthropology and Archeology, School of Social Sciences, National University of Mongolia, Mongolia.
- 5Department of Biology, Institute for Life Science, College of Natural Sciences, Kangwon National University, Korea.
- 6Department of Archaelogy and Art History, College of Humanities, Donga University, Korea.
- 7Division of Mathematical Science, College of Natural Sciences, Pukyong National University, Korea.
- 8DNA Analysis Section, National Institute of Scientific Investigation, Korea.
- 9Department of Laboratory Medicine, College of Medicine, Korea/
- 10Department of Philosophy, College of Liberal Arts, Chung-Ang University, Korea.
- 11Academy of Sciences of Republic of Uzbekistan, Uzbekistan.
- 12Department of Anatomy, College of Medicine, Chung-Ang University, Korea.
Abstract
- Determination of male and female is important in anthropology, archeology and forensic science. This study was designed to compare genotype sex of improved amelogenin PCR amplication method with morphological sex of ancient human bones. Sixty human skulls which lived from the Bronze Age to twenties centuries and excavated in Uzbekistan were used in this study. Morphological sex was determined by Uzbekistan scientist, and genotype sex was determined by improved amelogenin PCR amplication developed in this study. Among 20 morphological males, 13 samples (65%) were genotypical male. Among 40 morphological females, 20 samples (50%) were genotypical male. In conclusion, morphological method might be inadequate for sex determination of ancient bones. The improved amelogenin PCR method will be useful in sex determination of ancient bones.
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