Ann Clin Microbiol.  2014 Mar;17(1):14-19. 10.5145/ACM.2014.17.1.14.

Effects of Preincubating Blood Culture Bottles at 37degrees C during the Night Shift and of Collected Blood Volume on Time to Detection and Days to Final Report

Affiliations
  • 1Department of Laboratory Medicine, Gyeongsang Institute of Health Sciences, Gyeongsang National University School of Medicine, Jinju, Korea. sjkim8239@hanmail.net

Abstract

BACKGROUND
By varying the collected blood volume and storage temperature of the blood culture bottles prior to entry in an automated blood culture system, growth of organisms will be affected.
METHODS
Blood culture bottles with a 20 mL blood volume per set were stored at 37degrees C (1st period) and room temperature (RT, 2nd period) upon arrival at the laboratory after working hours compared to baseline period (10 mL, RT). The time to detection (TTD) for all strains and the number of days until the final report after bottle entry were compared among the three periods.
RESULTS
The median TTD for all strains was 13.5 h, 10.6 h, and 11.3 h in the baseline (N=268), 1st (N=454), and 2nd period (N=370), respectively (P<0.001). The final identification report was available within two days of bottle entry for 12.3%, 30.6% and 15.1% of bottles in the three different periods, respectively (P<0.001).
CONCLUSION
Collecting an adequate blood volume is critical to reduce TTD. The preincubation of blood culture bottles at 37degrees C during the night shift might enable earlier final reports than storage at RT for samples with the same collected blood volume.

Keyword

Blood culture; Detection; Storage

MeSH Terms

Blood Volume*

Reference

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