Effect of hyaluronic acid mixture on bone formation process of xenogeneic bone graft in rabbit

Min, Seung Ki; Hwang, Dong Hyeon; Cha, Soo Ryen; Kim, Young Seak; Kang, Moon Jeong

Abstract

It is known that hyaluronic acid is an essential growth component of extracellular matrices in early mesenchymal cells migration, proliferation, differentiation and binding to mamalian fibrinogen. Also, its gelation property is easy to handle, contour, and maintain the filling material like platelet rich plasma(PRP) or fibrin adhesive. This study was designed to evaluate whether hyaluronic acid mixture(HAM) has effects on bone formation process and capacity of clinical application on bony defect in substitution of PRP. Twenty four rabbit were included in this randomized, blinded, prospective pilot study. In experimental group, mandibular bone defects on the mandible whose size were 6 mm diameter round shape were created and immediately grafted with Bio-oss mixed with HAM. In control group, same bone defect were prepared and grafted with bio-oss with PRP on the other side. The specimens had been sacrificed at 1st, 2nd, 4th, 6th weeks postoperatively, proceeded for H and E and M/T staining and examined on light microscopic finding. At initial 1st, 2nd weeks, All group showed dense connection among grafted bone than control. At 4th week, all group showed that inflammatory response and bone resorption tendency were diminished, but in experimental group, osteoblastic activity and amount of bone formation were higher than control. After 2 weeks, all experimental and control group showed similar bone formation process and matured bone. Like above, Application of hyaluronic acid on bone grafting could have a sufficient capacity of substitute for PRP.