J Bacteriol Virol.  2007 Sep;37(3):169-175. 10.4167/jbv.2007.37.3.169.

Epidemiological Surveillance of P and G Genotypes of Group A Rotavirus Detected from Diarrheic Patients in Daejeon Region

• Park SK
• Heo YS
• Bing SH
• Cheon DS
• Chu J
• Jun MH

• KMID: 1513572
• DOI: http://doi.org/10.4167/jbv.2007.37.3.169

Abstract

During 3 years surveillance (January 2001 through December 2003) for acute gastroenteritis in human in Daejeon region, 432 out of 4,869 stool samples were selected as rotavirus-positive specimens by means of antigen-capture enzyme-linked immunosorbent assay (ELISA). The P (VP4) and G (VP7) genotypes for 432 stool samples were investigated by reverse transcription polymerase chain reaction (RT-PCR) and nested multiplex PCR. The most prevalent P subtype was P[8] (44.9%), followed by P[4] (25.7%) and P[6] (17.1%). No cases for P[10] and P[9] subtypes were found through the study. In G subtyping, G1 (53.2%) was the most frequently found G type, followed by G2 (23.1%), G3 (9.5%), G4 (6.7%), and G9 (0.9%). The order of detection rates for G2, G3 and G4 was variable by years. The most common G- and P- type combination found in this study was G1P[8] (33.1%), followed by G2P[4] (20.4%), G1P[6] (10.0%), G3P[8] (7.2%) and G4P[6] (4.2%). The mixed types of G and P were observed most frequently in P[8] (1.4%) and G1 (3.2%), respectively. This is the first molecular epidemiological study for Group A rotavirus in Daejeon region. The results might be useful data for evaluating the epidemiological status of rotaviral diarrhea in the region.

Keyword

Rotavirus; Acute gastroenteritis; P and G genotypes; Nested multiplex PCR

MeSH Terms

Diarrhea
Enzyme-Linked Immunosorbent Assay
Epidemiologic Studies
Gastroenteritis
Genotype*
Humans
Multiplex Polymerase Chain Reaction
Polymerase Chain Reaction
Reverse Transcription
Rotavirus*

Figure

• 

  Figure 1. Patterns of nested multiplex PCR for P and G genotypes of Group A rotavirus detected from stool specimens. (A) Lane M; 100 bp DNA ladder, Lanes 1; P[8], 2; P[4], 3: P[6]. (B) Lane M; 100 bp DNA ladder, Lanes 1; G1, 2; G2, 3; G3, 4; G9, 5; G4.

Reference

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