J Korean Assoc Oral Maxillofac Surg.  2008 Dec;34(6):635-639.

Effect of different sterilization methods on the surface morphology of PPDO-hybrid-PLGA nanofiber scaffold and attachments of PC12 cell

• Lee J
• Min H
• Jung J
• Kang N

Abstract

OBJECTIVES
the effect of different sterilization methods on the surface morphology of PPDO-hybrid-PLGA nanofiber scaffold and attachments of PC12 cell were investigated.
METHODS
Poly (p-dioxone)-hybrid-Poly (lactide-glycolide) (PPDO-hybrid-PLGA) nanofiber scaffold, fabricated in a tube form with 1.5 mm internal diameter, 0.2 mm thickness and 5 mm length, was prepared using electrospinning method. To study the surface morphology using SEM, The study group and control group in respective were; Control:Non-sterilized scaffold, Group I:scaffold sterilized with 70% Alcohol, Group II: scaffold sterilized with Ethylene Oxide at 65 degrees C, and Group III: scaffold sterilized with Ethylene Oxide at 37 degrees C. To investigate viability of the PC12 cell on the scaffold, The study group and control group in respective were; Control: sterilized with 70% Alcohol, Group I: sterilized with Ethylene Oxide at 65 degrees C, and Group II: sterilized with Ethylene Oxide at 37 degrees C.
RESULTS
1. The surface morphology was slightly changed in Group I, II and GroupIII, compared with control. 2. The attachment of PC12 cells in Group I, II was not higher than in control DISCUSSION: The attachment of PC12 cell is not influenced by different sterilization methods.

Keyword

Sterilization; Nanofiber scaffold; Surface morphology; Attachment of cell

MeSH Terms

Animals
Ethylene Oxide
Ethylenes
Nanofibers
PC12 Cells
Sterilization
Ethylene Oxide
Ethylenes