Korean J Thorac Cardiovasc Surg.  2009 Apr;42(2):157-164.

Effect of Diamine Bridges Using L-lysine in Glutaraldehyde Treated Porcine Pericardium

• Kim KC
• Choi YK
• Kim SH
• Kim YJ

Abstract

BACKGROUND: Various studies and experimental trials have been done to develop bioprosthetic devices to treat complex congenital heart disease due to the limited usage of homograft tissue. The purpose of the present study was to evaluate the effect of diamine bridges with using L-lysine, as compared with using ethanol. MATERIAL AND METHOD: Porcine pericardium was fixed at 0.625% GA (commercial fixation). An interim step of ethanol (80%; 1 day at room temperature) or L-lysine (0.1 M; 2 days at 37degrees C) was followed by completion of the GA fixation (2 days at 4degrees C and 7 days at room temperature). The tensile strength and thickness of the porcine percardium were measured, respectively. The treated pericardiums were implanted subcutaneously into three-week old Long-Evans rats for 8 weeks. The calcium content of the implants was assessed by atomic absorption spectroscopy and the histology. RESULT: Ethanol pretreatment (13.6+/-10.0 ug/mg, p=0.008), L-lysine pretreatment (15.3+/-1.0 ug/mg, p=0.002), and both treatment (16.1+/-11.1 ug/mg, p=0.012) significantly inhibited calcification, as compared with the controls (51.2+/-8.5 ug/mg). L-lysine pretreatment (0.18+/-0.02 mm, 1.20+/-0.30 kg f/5 mm) significantly increased the thickness and tensile strength, as compared with ethanol pretreatment (0.13+/-0.03 mm, 0.85+/-0.36 1.0 kg f/5 mm) (p<0.01, p=0.035).
CONCLUSION
The diamine bridges using L-lysine seemed to decrease the calcification of porcine pericardium fixed with glutaraldehyde, and this was comparable with Ethanol. Additionally, it seemed to enhance the thickness and tensile strength.

Keyword

Xenograft; Bioprosthesis; Calcification; Graft preservation; Pericardium

MeSH Terms

Absorption
Bioprosthesis
Calcium
Ethanol
Glutaral
Heart Diseases
Lysine
Pericardium
Rats, Long-Evans
Spectrum Analysis
Tensile Strength
Transplantation, Heterologous
Transplantation, Homologous
Calcium
Ethanol
Glutaral
Lysine