Korean J Clin Microbiol.  2009 Jun;12(2):97-101. 10.5145/KJCM.2009.12.2.97.

Central Venous Catheter-Related Microbacterium Bacteremia Identified by 16S ribosomal RNA Gene Sequencing

• Moon CJ
• Shin JH
• Jeong ES
• Kee SJ
• Kim SH
• Shin MG
• Suh SP
• Ryang DW

• KMID: 1469961
• DOI: http://doi.org/10.5145/KJCM.2009.12.2.97

Abstract

We describe here a case of central venous catheter (CVC)-related bacteremia caused by Microbacterium species in a 14-year-old patient, who had received chemotherapy for acute lymphoblastic leukemia. All nine blood cultures obtained from admission day 2 to day 62 yielded the same yellow-pigmented coryneform rod. Both Vitek 2 (bioMerieux, USA) and MicroScan (Dade Behring, USA) identified the isolate as Micrococcus species, and the API Coryne (bioMerieux, France) identified the isolate as Rhodococcus or Brevibacterium species. However, the 16S rRNA gene sequence showed a 99% identity with Microbacterium species. The bacteremia was recurrent or persistent over 60 days despite alternate systemic antibiotic therapy, but blood culture became negative after an addition of teicoplanin lock therapy for eradicating CVC-related bacteremia. This represents the first report of CVC-related Microbacterium bacteremia cured by antibiotic lock therapy in Korea.

Keyword

16S rRNA sequencing; Catheter-related bacteremia; Microbacterium species

MeSH Terms

Adolescent
Bacteremia
Brevibacterium
Central Venous Catheters
Genes, rRNA
Humans
Micrococcus
Precursor Cell Lymphoblastic Leukemia-Lymphoma
Rhodococcus
RNA, Ribosomal, 16S
Teicoplanin
RNA, Ribosomal, 16S
Teicoplanin