Lab Anim Res.  2011 Dec;27(4):365-368. 10.5625/lar.2011.27.4.365.

Antioxidative and aldose reductase-inhibitory effects of a fermentation filtrate of Rubus coreanus

• Kwon SC
• Kim YB

• KMID: 1444973
• DOI: http://doi.org/10.5625/lar.2011.27.4.365

Abstract

Antioxidative and aldose reductase (AR)-inhibitory effects of a fermentation filtrate of Rubus coreanus (FRC) were investigated using corneal/retinal homogenate and lens cytosol, respectively. Rat corneal/retinal homogenate was treated with 50 microM FeCl3 in the presence of FRC (3.2-100 microg/mL) for 30 min at 37degrees C, and thiobarbituric acid-reactive substances (TBARS) was quantified as a lipid peroxidation parameter. FRC markedly suppressed the TBARS production in a concentration-dependent manner, leading to 50% (IC50) and 100% (IC100) inhibitory concentrations of 20 and 95 microg/mL, respectively, which was similar to the effect of butylated hydroxyanisole. Activity of AR from rat lens was assayed in the presence of FRC (1-31.6 microg/mL) at 25degrees C using glyceraldehyde as a substrate. FRC inhibited lens AR by 50% (IC50) and 90% (IC90) at approximately 2 and 31.6 microg/mL, respectively, comparable to the effect of quercetin. The results indicate that ERC could be a promising candidate for the improvement of eye injury and visual dysfunction of dry eye and diabetic patients.

Keyword

Rubus coreanus; antioxidative activity; aldose reductase inhibition

MeSH Terms

Aldehyde Reductase
Animals
Butylated Hydroxyanisole
Cytosol
Eye
Eye Injuries
Fermentation
Glyceraldehyde
Humans
Lipid Peroxidation
Quercetin
Rats
Thiobarbituric Acid Reactive Substances
Aldehyde Reductase
Butylated Hydroxyanisole
Glyceraldehyde
Quercetin
Thiobarbituric Acid Reactive Substances

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