Korean J Pathol.  2011 Apr;45(2):182-187. 10.4132/KoreanJPathol.2011.45.2.182.

CellprepPlus(R) Liquid-based Smear in Sono-guided Thyroid Fine Needle Aspiration: A Comparison of Conventional Method and CellprepPlus(R) Liquid-based Cytology

• Koo JH
• Lee SY
• Lee HC
• Park JW
• Koong SS
• Oh TK
• Jeon HJ
• Kim EJ
• Lee OJ

• KMID: 1443752
• DOI: http://doi.org/10.4132/KoreanJPathol.2011.45.2.182

Abstract

BACKGROUND
Fine needle aspiration (FNA) of the thyroid is a useful tool for the evaluation of benign or malignant thyroid nodules. The improvements in the quality of cytological preparations using the liquid-based cytology (LBC) method have been well-documented. The principal objective of this study was designed to evaluate the diagnostic adequacy, sensitivity, and specificity of the thyroid FNA comparing a conventional smear with the LBC adapted with the filtration method described herein.
METHODS
One hundred ninety eight cases of FNA samples obtained from May 2009 to September 2009 were included in this study. All patients were subjected to ultrasound-guided aspiration twice at a target lesion by a radiologist and two types of slides were prepared using conventional smear and LBC.
RESULTS
When compared with conventional method, the cellularity was reduced in LBC. However, the malignant tumor cells evidenced the larger and more vesicular nuclei, prominent nucleoli, and distinct nuclear membranes in LBC. Thirty two cases (16.16%) of conventional smear were inadequate, but 96 cases (48.49%) of LBC were inadequate.
CONCLUSIONS
Most of the slides using CellprepPlus(R) LBC evidenced lower cellularity and clearer background. However, the conventional smears were found to generate much more applicable samples than CellprepPlus(R) LBC.

Keyword

Thyroid; Cytology; Aspiration

MeSH Terms

Biopsy, Fine-Needle
Filtration
Humans
Needles
Nuclear Envelope
Sensitivity and Specificity
Thyroid Gland
Thyroid Nodule