Korean J Pathol.  2011 Oct;45(5):485-490. 10.4132/KoreanJPathol.2011.45.5.485.

14-bp Insertion/Deletion Polymorphism of the HLA-G Gene in Osteosarcoma Patients

• Moon A
• Kim SK
• Chung JH
• Na KY
• Olvi LG
• Santini-Araujo E
• Kim YW
• Park YK

• KMID: 1443698
• DOI: http://doi.org/10.4132/KoreanJPathol.2011.45.5.485

Abstract

BACKGROUND
The major histocompatibility complex class I, G (human leukocyte antigen-G [HLA-G]) gene plays a vital role in the suppression of immune responses. Recently, a number of studies have reported an association between HLA-G and diseases (pregnancy complications, organ transplantation, and tumors). Some of the studies have revealed that the 14-bp insertion/deletion polymorphism might be associated with various diseases. The aim of the present study was to explore a possible influence of the 14-bp insertion/deletion polymorphism on osteosarcoma.
METHODS
Genomic DNA was extracted from 75 formalin-fixed, paraffin-embedded tumor tissues derived from patients with conventional osteosarcoma (OSA) and 183 peripheral blood samples of healthy controls. Fifty-eight cases were South Korean patients with OSA and 17 cases were Argentine patients with OSA. The HLA-G 14-bp insertion/deletion polymorphism at exon 8 of the HLA-G locus was analyzed by polymerase chain reaction.
RESULTS
There was a significantly different distribution profile for the 14-bp genotypes between the Korean OSA and Korean control groups. Specifically, there were more heterozygote 210 bp/224 bp genotypes in the Korean OSA group when compared to the Korean control group (62.1% vs 40.4%, p=0.002).
CONCLUSIONS
The results suggest that HLA-G heterozygote patients may be more susceptible to OSA in the Korean population.

Keyword

HLA-G; 14-bp insertion/deletion polymorphism; Osteosarcoma

MeSH Terms

DNA
Exons
Genotype
Heterozygote
HLA-G Antigens
Humans
Leukocytes
Major Histocompatibility Complex
Organ Transplantation
Osteosarcoma
Transplants
DNA
HLA-G Antigens