Korean J Pediatr Infect Dis.  2012 Aug;19(2):55-60.

Immunoassay of Pertussis According to Ages

Affiliations
  • 1Department of Pediatrics, The Catholic University of Korea, Seoul, Korea. kjhan@catholic.ac.kr
  • 2Department of Internal Medicine, The Catholic University of Korea, Seoul, Korea.
  • 3Department of Pediatrics, Changwon Fatima Hospital, Changwon, Korea.
  • 4Department of Pediatrics, Soon Cheon Hyang University, Cheonan, Korea.
  • 5Department of Pediatrics, Wonju Yonsei University, Wonju, Korea.

Abstract

PURPOSE
We conducted the immunoassay of pertussis according to ages, in order to evaluate protective immunity against pertussis in Korean populations.
METHODS
Healthy subjects were enrolled at four university hospitals in Korea. The subjects were grouped as seven age groups (every 10 years). Antibodies against pertussis toxin (PT) in sera were measured by enzyme linked immunosorbent assay (ELISA) kits. Geometric mean concentrations (GMC) of antibodies and the ratios of the subjects with seroprotective antibody levels were determined. The subjects with antibody titers > or =24.0 EU/mL were considered to seroprotective as the manufacturer's protocol.
RESULTS
Total 1,605 subjects (age: 2 months-65 years) participated in this study, and their GMC was 56.16+/-50.54 EU/mL. Among seven age groups, age group <11 year showed the highest GMC (64.78+/-53.24 EU/mL) (P<0.001). In the analysis of the ratios of the subjects with seroprotective antibody titers, 68.2% of the subjects were proven to seroprotective, and age group <11 year also showed the highest ratio (76.5%) (P<0.001).
CONCLUSIONS
We found that adolescences or adults (age group > or =11 year) showed lower levels of antibody against pertussis and lower ratio of the subjects with seroprotective antibody titers than children (age group <11 year).

Keyword

Pertussis; Immunoassay; Immunization; Booster

MeSH Terms

Adult
Antibodies
Child
Enzyme-Linked Immunosorbent Assay
Hospitals, University
Humans
Immunization
Immunoassay
Korea
Pertussis Toxin
Whooping Cough
Antibodies
Pertussis Toxin

Figure

  • Fig. 1 Geometric mean concentrations (GMC) of subjects' antibodies against pertussis toxin (PT) are shown by age group and sex. (A) Adolescents and adults, and (B) children. Data of the past study performed in 200214) are also shown to compare the differences.


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