J Lab Med Qual Assur.  2012 Dec;34(2):87-92.

Comparison of Six Clinical Chemistry Test Results According to the Treatment of EDTA Anticoagulant in Body Fluid Specimens

• Lee D
• Jang JY
• Yoon KR
• Kim H
• Uh Y
• Kim J
• Yoon KJ

Abstract

BACKGROUND
Specimen requirements such as type of anticoagulant and number of tube for body fluid analysis vary with specimen type and requested laboratory tests. We compared the results of six clinical chemistry tests between EDTA anticoagulated and anticoagulant-free body fluids.
METHODS
A total of 191 body fluids (45 pleural, 28 bronchoalveolar lavage, 35 peritoneal, 45 peritosol, and 38 synovial fluids) were aliquoted into EDTA tubes and anticoagulant-free tubes, and were simultaneously tested for total protein, albumin, glucose, lactate dehydrogenase, adenosine deaminase, and amylase.
RESULTS
The coefficient of determination (R2) for all six clinical chemistry test results between EDTA anticoagulated and anticoagulant-free body fluids are more than 0.95 with the exception of glucose in bronchoalveolar lavage fluid (R2= 0.78).
CONCLUSIONS
EDTA anticoagulated specimen could be used for testing routinely requested clinical chemistry tests in body fluid analysis, that only one tube of specimen is necessary to perform cell count, differential count, and clinical chemistry tests.

Keyword

Body fluids; Clinical chemistry tests; Anticoagulants

MeSH Terms

Adenosine Deaminase
Anticoagulants
Body Fluids
Bronchoalveolar Lavage
Bronchoalveolar Lavage Fluid
Cell Count
Chemistry, Clinical
Clinical Chemistry Tests
Edetic Acid
Glucose
L-Lactate Dehydrogenase
Adenosine Deaminase
Anticoagulants
Edetic Acid
Glucose
L-Lactate Dehydrogenase