J Korean Med Sci.  1993 Oct;8(5):361-366. 10.3346/jkms.1993.8.5.361.

Immunohistochemical analysis of p53 protein expression in benign and malignant skin tumors using a panel of anti-p53 antibodies

• Ro YS
• Kim JH

• KMID: 1422004
• DOI: http://doi.org/10.3346/jkms.1993.8.5.361

Abstract

The expression of p53 in a variety of benign and malignant skin lesions has been first assessed in frozen sections and then compared with the results obtained in corresponding paraffin-embedded sections using various immunohistochemical staining methods with a panel of anti-p53 antibodies. Of the 48 benign and malignant skin lesions studied, 46(96%) had corresponding paraffin sections and immunohistochemical results obtained with DO7 on frozen and paraffin sections were concordant in 97%, qualitatively. Using streptavidin-biotin complex method, p53 was identified in 33% of dysplastic squamous lesions, 50% of squamous cell carcinomas (SCCs) and 36% of basal cell carcinomas (BCCs) on frozen section, whereas 25% of dysplastic squamous lesions, 40% of SCCs, and 32% of BCCs showed p53 positivity on paraffin-embedded sections. In frozen sections, the same regions of each specimen exhibited similar topographic patterns of positive immunoreactivity with both monoclonal antibodies, PAb 1801 and DO7. In contrast, immunohistochemical staining with polyclonal antibody, CM-1, gave poor morphologic resolution, although effective in paraffin-embedded sections.

Keyword

Immunohistochemistry; p53; skin tumors

MeSH Terms

Animals
Antibodies, Monoclonal/*immunology
Carcinoma, Basal Cell/chemistry
Humans
Immunohistochemistry
Mice
Skin Neoplasms/*chemistry
Staining and Labeling
Tumor Suppressor Protein p53/*analysis/immunology
Antibodies, Monoclonal
Tumor Suppressor Protein p53