J Korean Med Sci.  1991 Mar;6(1):15-19. 10.3346/jkms.1991.6.1.15.

Experimental orthotopic penetrating keratoplasty--a rat penetrating keratoplasty model

• Tchah H
• Youn DH
• Holland EJ

• KMID: 1421510
• DOI: http://doi.org/10.3346/jkms.1991.6.1.15

Abstract

An orthotopic penetrating keratoplasty model was developed in the rat. An oversized (0.5 mm) graft was used and 8 interrupted sutures were applied. These sutures were not removed. Eleven grafts out of 13 were rejected by the 3rd week in the disparate group (Brown Norway rat to Lewis rat transplantation group), which was characterized by edema, opacity, and neovascularization. All grafts remained clear in the syngeneic group (Lewis rat to Lewis rat transplantation group). Immunohistochemical examination was performed. This model seems to be a reliable and reproducible one to evaluate rejection reaction in corneal transplantation.

Keyword

Animal model; Penetrating keratoplasty; Rat; Orthotopic; Rejection reaction

MeSH Terms

Animals
Female
Graft Rejection
Keratoplasty, Penetrating/immunology/*methods/pathology
Lymphocyte Subsets/immunology/pathology
Macrophages/immunology/pathology
Rats
Rats, Inbred BN
Rats, Inbred Lew
Transplantation, Homologous
Transplantation, Isogeneic