J Korean Med Sci.  1990 Mar;5(1):13-17. 10.3346/jkms.1990.5.1.13.

The Enhancing Effect of Ethanol on the Development of Glutatione S-Transferase Placental Form-Positive Foci Induced by Diethylnitrosamine in F344 Rat

• Cho KJ
• Myung NH
• Jang JJ

• KMID: 1421473
• DOI: http://doi.org/10.3346/jkms.1990.5.1.13

Abstract

The effects of ethyl alcohol and pig serum administration on the development of preneoplastic hepatic enzyme-altered foci were examined in an in vivo mid-term assay system. Rats were initially given a single dose (200 mg/Kg) intraperitoneal injection of diethylnitrosamine (DEN). Two weeks later, treatment was started with 10% ethanol + 10% sucrose solution, 10% sucrose solution, or tap water as drinking water for 6 weeks with or without intraperitoneal injection of porcine serum twice a week. All rats were subjected to a two-thirds partial hepatectomy at week 3. The modification potentials were evaluated by comparing the number and area per cm2 of glutathione S-transferase placental form-positive (GST-P+) foci in the liver of each group. As a result, ethanol significantly enhanced the development of GST-P+ foci. Unfortunately, the porcine serum injection produced no hepatic fibrosis and no significant alteration in GST-P+ foci.

Keyword

Ethanol; Porcine serum; DEN; GST-P; foci

MeSH Terms

Animals
Diethylnitrosamine/*toxicity
Ethanol/*pharmacology
Glutathione Transferase/*metabolism
Immune Sera/pharmacology
Liver Cirrhosis, Alcoholic/enzymology
Male
Placenta/drug effects/*enzymology
Precancerous Conditions/*chemically induced/enzymology
Rats
Rats, Inbred F344
Survival Rate
Swine
Immune Sera
Diethylnitrosamine
Ethanol
Glutathione Transferase