J Korean Med Sci.  2012 May;27(5):576-577. 10.3346/jkms.2012.27.5.576.

Letter to the Editor: Detection of EML4-ALK and Other ALK Fusion Genes in Lung Cancer: A Lesson from the Leukemia Fusion Gene Analysis and Future Application

• Park TS
• Jeon YL
• Lee HJ
• Jeong JH
• Kim SY
• Cho EH
• Marschalek R
• Meyer C

• KMID: 1372806
• DOI: http://doi.org/10.3346/jkms.2012.27.5.576

Abstract

No abstract available.

MeSH Terms

Asian Continental Ancestry Group/*genetics
Carcinoma, Non-Small-Cell Lung/*genetics
Female
Humans
Lung Neoplasms/*genetics
Male
Oncogene Proteins, Fusion/*genetics

Figure

• 

  Fig. 1 Schematic representation of long distance inverse-polymerase chain reaction (LDI-PCR) for analyzing ALK rearrangements. LDI-PCR can analyze any kind of EML4-ALK fusion variant and other (or unknown) ALK partner genes. (A) One ALK wildtype allele and one rearranged ALK allele are presented. Genomic breakpoint cluster region (BCR) of ALK in non-small cell lung cancer (NSCLC) is located in 19th intron of ALK gene. (R: restriction enzyme) (B) General principle of LDI-PCR for the detection of ALK fusion gene analysis. Two asterisks show the derivative (target) bands by LDI-PCR in ALK fusion gene analysis. (C) Demonstration of known (EML4, TFG, KIF5B, KLC1) or unknown partner genes in ALK rearrangements.

Reference

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