J Vet Sci.  2011 Dec;12(4):353-361. 10.4142/jvs.2011.12.4.353.

Genotypic characterization of Staphylococcus aureus isolated from bovines, humans, and food in Indonesia

• Salasia SI
• Tato S
• Sugiyono N
• Ariyanti D
• Prabawati

• KMID: 1365019
• DOI: http://doi.org/10.4142/jvs.2011.12.4.353

Abstract

The present study determined the genetic relationships between 41 Staphyloccocus (S.) aureus isolates from bovines, humans, and food using a single enzyme amplified fragment length polymorphism (AFLP) technique. We evaluated the prevalence of staphylococcal enterotoxin (SE) genes and other virulence gene determinants by PCR. The identification of S. aureus was based on culturing and biochemical tests, and by amplifying a specific section of the 23S rRNA gene. PCR amplification of the SE genes (sea, seb, sec, see, seg, seh, and sei) singly or in combination was observed. Most isolates of bovine origin harbored hla (84%) and cap5 (74%), while most isolates from humans harbored hla (73%), cap8 (91%), and fnbA (100%). Strains from food sources were positive for hla (100%), cap5 (100%), and cap8 (64%) unlike isolates from humans or bovines. A single enzyme AFLP analysis revealed a correlation between AFLP clusters of some strains and the source of the isolates The genotypic results of the present study might help to better understand the distribution of prevalent S. aureus clones among humans, bovines, and food and will help control S. aureus infections in Indonesia.

Keyword

amplified fragment length polymorphism; enterotoxin; genetic determinant; Staphylococcus aureus

MeSH Terms

Amplified Fragment Length Polymorphism Analysis
Animals
Bacterial Proteins/genetics/metabolism
Cattle
Female
*Food Microbiology
Gene Expression Regulation, Bacterial
Humans
Indonesia/epidemiology
Mastitis, Bovine/epidemiology/microbiology
Phylogeny
Staphylococcal Infections/epidemiology/microbiology/*veterinary
Staphylococcus aureus/*genetics

Figure

• 

  Fig. 1 Agarose gel electrophoresis of se gene PCR products including sei (Lane 1, 576 bp), seh (Lane 2, 478 bp), seb (Lane 3, 375 bp), a negative enterotoxine strain (Lane 4), and sea (Lane 5, 216 bp). M: marker = 100 bp DNA ladder.

• 

  Fig. 2 Dendrogram of amplified fragment length polymorphism (AFLP) patterns showing the relatedness of the 41 Staphylococcus aureus strains examined in this study. Degrees of similarity were calculated using BioNumerics software with the Dice coefficient and cluster analysis with UPGMA. *AFLP patterns A to X (23 types). †Clusters of strains identified by a cutoff value of 0.25 (I to IX). ‡Strain designations: B; bovine source, F; food source, H; human source.

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