Immunohistochemical Analysis for the Expression of p27, CDK4, and Uteroglobin in Resected Non-small Cell Lung Cancer

Baek, Man Sun; Hong, Seok Gyun; Hong, Hyun Ju; Um, Sang Won; Lee, Kye Young; Kim, Woo Ho; Lee, Choon Taek; Yoo, Chul Gyu; Han, Sung Koo; Shim, Young Soo; Kim, Young Whan

Abstract

BACKGROUND: It has been reported that the expression of protein which influences on the cell cycle is significantly involved in the development, progress, treatment response, and survival of cancer, and also that the degree of expression of p27 and CDK4 is related to the prognosis. Recent research has revealed that uteroglobin, tumor suppressor gene, is related to cell cycle. This study is focused on the relations between expression of proteins related to cell cycle and clinical index of and survival of NSCLC. METHODS: We examined immunohistochemically specimens of 110 surgically resected NSCLCs for expression of p27, CDK, Uteroglobin. Tissue array slide were obtained from 110 surgically resected NSCLCs. Immunohistochemical staining was performed by immuno-peroxidase technique using avidin-biotinylated horseradish peroxidase complex. RESULTS: In 110 patients with resected NSCLCs, the ratio of male to female was 87:13, the median age was 56.43+/-9.41 yrs. The positive staining of p27 was detected in 75% of the cases. A non-statistically significant trend toward increased p27 expression was observed in smoker and squamous cell cancer. The positive staining of CDK4 was detected in 89%, which was the highest expression of protein among 3 types. The survival ratio of CDK4 negative staining group was higher than that of positive staining group, which was significant difference(P<0.05). There was no association between p27 or uteroglobin expression and survival. CONCLUSIONS: The expression degree of CDK4 is related to the prognosis. This findings suggests that the measurement of CDK4 may be useful in identifying patient at high risk for disease recurrence and survival.