Helicobacter pylori cagA Gene and Host Interleukin-1 Beta and Interleukin-1 Receptor Antagonist Gene Polymorphisms in Gastric Carcinoma

Lee, Jae Seung; Kim, Gwang Ha; Heo, Jeong; Park, Do Youn; Jun, Eun Sook; Kang, Dae Hwan; Song, Geum Am; Cho, Mong; Yang, Ung Suk

Abstract

BACKGROUND/AIMS: Both Helicobacter pylori (H. pylori) cagA genotype and host interleukin-1 beta/interleukin-1 receptor antagonist (IL-1B/IL-1RN) gene polymorphisms play a role in determining the clinical consequences of H. pylori infection. In this study, we investigated H. pylori cagA genotype and host IL-1B/IL-1RN polymorphisms in Korean patients to clarify their involvement in the occurrence of gastric carcinoma. METHODS: The population comprised of 134 patients with H. pylori infection: 82 with gastric carcinoma and 52 with gastritis. The DNA was isolated from gastric biopsy sample and H. pylori cagA genotype was determined by PCR. The IL-1B-511 polymorphism was genotyped by PCR-RFLP and the IL-1RN polymorphism was analyzed. RESULTS: Infection with cagA+ H. pylori was not associated with an increased risk for gastric carcinoma. The IL-1B-511 *T carriers and IL-1RN *2 carriers did not show an increased risk for gastric carcinoma. Combining bacterial/host genotypes, cagA+/IL-1B-511 *T carriers and cagA+/IL-1RN *2 carriers showed no increased risk of gastric carcinoma. Classifying of gastric carcinoma into intestinal and diffuse type, the bacterial/host genotypes were also not associated with increased risk of each type. CONCLUSIONS: Combined H. pylori cagA gene and host IL-1B/IL-1RN polymorphisms showed no increased risk of gastric carcinoma. Therefore, it seems other endogenous or exogenous factors might play more important roles in the development of gastric carcinoma in Korean.