J Korean Med Sci.  2003 Oct;18(5):637-640. 10.3346/jkms.2003.18.5.637.

Detrimental Effects of N(w)-nitro-L-arginine Methyl Ester (L-NAME) in Experimental Escherichia coli Sepsis in the Newborn Piglet

• Kim SS
• Hwang JH
• Choi CW
• Shim JW
• Chang YS
• Park WS
• Oh CK

• KMID: 1130031
• DOI: http://doi.org/10.3346/jkms.2003.18.5.637

Abstract

The role of nitric oxide during neonatal sepsis is complex. We tested the hypothesis that nonselective inhibition of nitric oxide synthase with N(w) -nitro-L-arginine methyl ester (L-NAME) is detrimental during the early phase of experimental sepsis in the newborn piglet. Newborn piglets were divided into four groups: 6 in the control group, 6 in the L-NAME control group, 12 in the sepsis group (SG), and 11 in the sepsis with L-NAME group (NS). Sepsis was induced by intravenous injection of 10(8) colony forming units of Escherichia coli. L-NAME 10 mg/kg was given intravenously 60 min before the induction of sepsis. The survival rate of piglets after 4 hr was 27% in NS, while it was 100% in other groups. Systemic hypotension, observed in both SG and NS, were more profound in NS. Leukopenia was observed in both SG and NS. Thrombocytopenia, prolongation of prothrombin time and activated partial thromboplastin time, and increase in thrombin-antithrombin complexes were observed only in NS. Decreased PaO2 /FiO2 ratio, arterial pH and base excess, and increased blood lactate levels observed in both SG and NS, but were more profound in NS. These findings suggest that nonselective inhibition of nitric oxide synthase with L-NAME is detrimental during the early phase of experimental neonatal sepsis.

Keyword

Sepsis; Nitric Oxide; Animals, Newborn; Escherichia coli; NG-Nitroarginine Methyl Ester

MeSH Terms

Animals
Animals, Newborn
Enzyme Inhibitors/pharmacology
Escherichia coli/*metabolism
NG-Nitroarginine Methyl Ester/*pharmacology
Sepsis/*drug therapy
Support, Non-U.S. Gov't
Swine
Time Factors