J Korean Med Sci.  2002 Oct;17(5):599-603. 10.3346/jkms.2002.17.5.599.

Mutations in the 23S rRNA Gene of Helicobacter pylori Associated with Clarithromycin Resistance

• Kim KS
• Kang JO
• Eun CS
• Han DS
• Choi TY

• KMID: 1127248
• DOI: http://doi.org/10.3346/jkms.2002.17.5.599

Abstract

Among 12 clarithromycin-resistant Helicobacter pylori strains isolated in Guri, Korea, 8 showed an adenine to guanine mutation at position 2143 (formerly A2144G or E. coli 2059) in the 23S rRNA gene by the PCR-restriction fragment length polymorphism (RFLP) method. The remaining 4 strains, digested by neither BsaI nor BbsI, showed a thymine to cytosine mutation at position 2182 (T2182C) by direct sequencing of the PCR products. The T2182C mutants showed a tendency of higher levels of minimum inhibitory concentration to clarithromycin than the A2143G mutants. In conclusion, either the A2143G or the T2182C mutation was present in 100% of clarithromycin-resistant H. pylori isolates examined. The PCR-RFLP technique with restriction enzymes BbsI and BsaI was a rapid and relatively simple method to detect the clarithromycin resistance. But undigested isolates were quite frequent among our isolates (33.3%), the PCR-RFLP method with restriction enzymes BbsI and BsaI should not be used alone, and development of other rapid detection method for clarithromycin resistance is mandatory.

Keyword

Helicobacter pylori; Clarithromycin; Resistance; 23S rRNA Gene; Mutation

MeSH Terms

Amino Acid Sequence
Anti-Bacterial Agents/*pharmacology
Base Sequence
Clarithromycin/*pharmacology
DNA, Bacterial/genetics
Drug Resistance, Bacterial/genetics
*Genes, Bacterial
Helicobacter Infections/drug therapy/microbiology
Helicobacter pylori/*drug effects/*genetics
Humans
Korea
Molecular Sequence Data
Mutation
RNA, Bacterial/genetics
RNA, Ribosomal, 23S/genetics
Sequence Homology, Nucleic Acid