Exp Mol Med.  2005 Dec;37(6):619-623.

CKbeta8-1 alters expression of cyclin E in colony forming units-granulocyte macrophage (CFU-GM) lineage from human cord blood CD34 + cells

• Noh EK
• Ra JS
• Lee SA
• Kwon BS
• Han IS

Abstract

A C6 beta-chemokine, CKbeta8-1, suppressed the colony formation of CD34 + cells of human cord blood (CB). Molecular mechanisms involved in CKbeta8-1-medicated suppression of colony formation of CD34 + cells are not known. To address this issue, the level of various G1/S cell cycle regulating proteins in CKbeta8-1-treated CD34 + cells were compared with those in untreated CD34 + cells. CKbeta8-1 did not significantly alter the expression of the G1/S cycle regulation proteins (cyclin D1, D3, and E), CDK inhibitor (p27and Rb), and other cell proliferation regulation protein (p53) in CB CD34 + cells. Here we describe an in vitro system in which CB CD34 + cells were committed to a multipotent progenitor lineage of colony forming units-granulocyte/macrophage (CFU-GM) by a simple combination of recombinant human (rh) GM-CSF and rhIL-3. In this culture system, we found that cyclin E protein appeared later and disappeared faster in the CKbeta8-1-treated cells than in the control cells during CFU-GM lineage development. These findings suggested that cyclin E may play a role in suppressing the colony formation of CFU-GM by CKbeta8-1.

Keyword

cell cycle; chemokine; cyclin E; granulocytes; macrophages; multi potent; stem cells

MeSH Terms

Antigens, CD34/metabolism
Cell Cycle Proteins/metabolism
Cell Lineage
Cells, Cultured
Chemokines, CC/*pharmacology
Cyclin E/*metabolism
Fetal Blood/*cytology
G1 Phase/drug effects
Gene Expression Regulation/*drug effects
Granulocytes/cytology/*drug effects/metabolism
Growth Substances/pharmacology
Humans
Macrophages/cytology/*drug effects/metabolism
Research Support, Non-U.S. Gov't
Stem Cells/cytology/*drug effects/metabolism