Exp Mol Med.  1998 Sep;30(3):145-149.

Cloning of adriamycin-resistant related (arr) gene in an adriamycin-resistant L1210 variant

Affiliations
  • 1Department of Biochemistry, College of Medicine, Yeungnam University, Daegu, Korea.

Abstract

A partial fragment of novel sequence (arr, adriamycin-resistant related) was previously identified using the differential display (DD)-PCR technique with adriamycin-resistant L1210 variant (L1210AdR), which shows a typical multidrug resistant (MDR) phenotypes. The present research shows the isolation of full length arr cDNA sequence. To clone the full length cDNA of arr gene, DD-PCR fragments were subjected to 5'- and 3'-Rapid Amplification of cDNA End (RACE) method. The cloned arr cDNA consisted of 770 bases and contained an open reading frame of 153 bases, encoding a protein of 51 amino acid with the molecular mass of 4 kDa by in vitro translation reactions. Northern blot analysis showed that a 770 bases transcript arr gene was overexpressed in adriamycin-resistant L1210 variant, but not in the parent suggesting that the arr gene may be involved in the adriamycin-resistant phenotypes.

Keyword

MDR; arr; DD-PCR; RACE

MeSH Terms

Amino Acid Sequence
Animal
Base Sequence
Blotting, Northern
Cloning, Molecular
DNA Primers
Doxorubicin/pharmacology*
Drug Resistance, Neoplasm/genetics*
Gene Expression Regulation, Neoplastic
Glycoproteins/genetics*
Leukemia L1210/genetics*
Leukemia L1210/drug therapy
Mice
Molecular Sequence Data
Translation, Genetic
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