J Vet Sci.  2002 Sep;3(3):175-178.

Immunohistochemistry of Voltage-Gated Calcium Channel alpha1B Subunit in Mouse Cerebellum

• Chung SH
• Lee KY
• Kim KH
• Kim CT
• Lee NS
• Sawada K
• Haga H
• Lee BC
• Fukui Y
• Rhee MH
• Jeong YG

Abstract

Secretion of neurotransmitters is initiated by voltagegated calcium influx through presynaptic, voltage- gated N-type calcium channels. However, little is known about their cellular distribution in the mouse cerebellum. In the cerebellum, alpha1B immunoreactivity is found mainly on the cell bodies of all Purkinje cells. In addition, the immunoreactivity was detected on a subset of Purkinje cell dendrites, clustered to form a parasagittal array of bands. In the anterior lobe vermis, immunoreactive Purkinje cell dendrites form narrow stripes separated by broad bands of unstained dendrites. Moving caudally through the vermis, these stripes become thicker as a larger fraction of the Purkinje cell dendrites become immunoreactive. This localization study of the alpha1B pore-forming subunits in mouse cerebellum may guide future investigations of the role of calcium channels in neurological pathways.

Keyword

N-type calcium channels; Purkinje cell; Cerebellum

MeSH Terms

Animals
Calcium Channels, N-Type/*metabolism
Cerebellum/cytology/*metabolism
Dendrites/metabolism
Immunohistochemistry
Mice
Mice, Inbred BALB C
Purkinje Cells/metabolism