Yonsei Med J.  1993 Sep;34(3):258-265. 10.3349/ymj.1993.34.3.258.

Effect of polyadenylic.polyuridylic acid on cellular responses of peripheral blood mononuclear cells from patients with chronic active hepatitis B

• Kim WH
• Hahm KB
• Park SJ
• Kang JK
• Park IS
• Choi HJ
• Shin JS
• Youn JK

• KMID: 1092419
• DOI: http://doi.org/10.3349/ymj.1993.34.3.258

Abstract

We have investigated in vitro proliferative responses of peripheral blood mononuclear cells and productions of interferon-gamma and soluble interleukin-2 receptors by these cells from 6 patients with chronic active hepatitis B immediately before and 24 hours after a single intravenous injection of 100 mg of polyadenylic.polyuridylic acid. Cell proliferations were assessed by the technique of tritiated-thymidine incorporation and productions of interferon-gamma and soluble interleukin-2 receptors were measured by enzyme-linked immunosorbent assay. The administration of polyadenylic.polyuridylic acid to the patients has resulted in significant increases of in vitro proliferations of their peripheral blood mononuclear cells as well as productions of interferon-gamma by these cells. However, in vitro productions of soluble interleukin-2 receptors were not changed significantly. These results suggest that the enhanced cellular responses by polyadenylic.polyuridylic acid might be due to the increased sensitivity rather than the increased expression of cellular interleukin-2 receptor.

Keyword

Polyadenylic.polyuridylic acid; chronic active hepatitis B; cytokine

MeSH Terms

Adult
Hepatitis B/*immunology
Hepatitis, Chronic/*immunology
Human
Immunity, Cellular/drug effects
Interferon Type II/biosynthesis
Leukocytes, Mononuclear/*drug effects/immunology
Male
Middle Age
Poly A-U/*pharmacology
Receptors, Interleukin-2/biosynthesis
Solubility