Korean J Parasitol.
2011 Jun;49(2):195-197. 10.3347/kjp.2011.49.2.195.
Molecular Identification of Taenia Tapeworms by Cox1 Gene in Koh Kong, Cambodia
- Affiliations
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- 1Department of Parasitology and Medical Research Institute, Chungbuk National University School of Medicine, Cheongju 361-711, Korea. kseom@chungbuk.ac.kr
- 2Department of Parasitology and Institute of Tropical Medicine, Yonsei University College of Medicine, Seoul 120-752, Korea.
- 3Department of Parasitology and Institute of Health Sciences, Gyeongsang National University College of Medicine, Jinju 660-702, Korea.
- 4Department of Parasitology and Tropical Medicine, Seoul National University College of Medicine, Seoul 110-799, Korea.
- 5Department of Medical Environmental Biology, Chung-Ang University College of Medicine, Seoul 156-756, Korea.
- 6Department of Parasitology, Gangwon University College of Medicine, Chuncheon 200-722, Korea.
- 7International Cooperation Department, Korea Association of Health Promotion, Seoul 157-704, Korea.
- 8National Center for Parasitology, Entomology & Malaria Control, Phnom Penh, Cambodia.
- KMID: 1090624
- DOI: http://doi.org/10.3347/kjp.2011.49.2.195
Abstract
- We collected fecal samples from 21 individuals infected with Taenia tapeworms in Koh Kong Province, Cambodia, and performed nucleotide sequencing of the cox1 gene and multiplex PCR on the eggs for DNA differential diagnosis of human Taenia tapeworms. Genomic DNA was extracted from the eggs of a minimum number of 10 isolated from fecal samples. Using oligonucleotide primers Ta7126F, Ts7313F, Tso7466F, and Rev7915, the multiplex PCR assay proved useful for differentially diagnosing Taenia solium, Taenia saginata, and Taenia asiatica based on 706, 629, and 474 bp bands, respectively. All of the Taenia specimens from Kho Kong, Cambodia, were identified as either T. saginata (n=19) or T. solium (n=2) by cox1 sequencing and multiplex PCR.
Keyword
MeSH Terms
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Adolescent
Adult
Animals
Cambodia
Child
Cyclooxygenase 1/*genetics
DNA Primers/genetics
DNA, Helminth/chemistry/genetics
Feces/parasitology
Female
Helminth Proteins/*genetics
Humans
Male
Middle Aged
Polymerase Chain Reaction
Sequence Analysis, DNA
Taenia saginata/*enzymology/*genetics/isolation & purification
Taenia solium/*enzymology/*genetics/isolation & purification
Taeniasis/*parasitology
Young Adult
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