Korean J Parasitol.  2008 Jun;46(2):59-63. 10.3347/kjp.2008.46.2.59.

Expressed Sequence Tags of Trichinella spiralis Muscle Stage Larvae

Affiliations
  • 1Department of Internal Medicine, Pusan National University Hospital Medical Research Institute, Busan 602-739, Korea.
  • 2Department of Parasitology, School of Medicine, Pusan National University, Busan 609-735, Korea. hsyu@pusan.ac.kr
  • 3Pusan National University Hospital Medical Research Institute, Busan 602-739, Korea.
  • 4Department of Parasitology and Malariology, PICR, College of Medicine and Frontier Inje Research for Science and Technology, Inje University, Busan 614-735, Korea.
  • 5Department of Parasitology, School of Medicine, Kyungpook National University, Daegu 700-422, Korea.

Abstract

In order to obtain greater insight into the relevant genomic expression patterns of Trichinella spiralis, 992 expressed sequence tags (ESTs) were collected from a cDNA library of T. spiralis muscle stage larvae and assembled into 60 clusters and 385 singletons. Of them, 445 (44.7%) ESTs were annotated to their homologous genes, and small fractions were matched to known genes of nematodes. The annotated ESTs were classified into 25 eukaryotic orthologous groups (KOG). Cytochrome C oxidase (34 clones) was found to be most frequent species.

Keyword

Trichinella spiralis ; Expressed sequence tags; muscle stage larva; eukaryotic orthologous groups (KOG)

MeSH Terms

Animals
*Expressed Sequence Tags
Gene Library
Helminth Proteins/genetics
Larva/*genetics
Muscle, Skeletal/*parasitology
Rats
Trichinella spiralis/*genetics/isolation & purification
Trichinosis/*parasitology
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