J Vet Sci.  2010 Dec;11(4):277-283. 10.4142/jvs.2010.11.4.277.

Histopathological retrospective study of canine renal disease in Korea, 2003~2008

• Yhee JY
• Yu CH
• Kim JH
• Im KS
• Chon SK
• Sur JH

• KMID: 1072175
• DOI: http://doi.org/10.4142/jvs.2010.11.4.277

Abstract

Renal disease includes conditions affecting the glomeruli, tubules, interstitium, pelvis, and vasculature. Diseases of the kidney include glomerular diseases, diseases of the tubules and interstitium, diseases of renal pelvis, and developmental abnormalities. Renal tissue samples (n = 70) submitted to the Department of Veterinary Pathology of Konkuk University from 2003 to 2008 were included in this study. Tissue histopathology was performed using light microscopy with hematoxylin and eosin stains. Masson's trichrome, Congo Red, and Warthin starry silver staining were applied in several individual cases. Glomerular diseases (22.9%), tubulointerstitial diseases (8.6%), neoplastic diseases (8.6%), conditions secondary to urinary obstruction (24.3%), and other diseases (35.7%) were identified. Glomerulonephritis (GN) cases were classified as acute proliferative GN (5.7%), membranous GN (4.3%), membranoproliferative GN (4.3%), focal segmental GN (2.9%), and other GN (4.2%). The proportion of canine GN cases presently identified was not as high as the proportions identified in human studies. Conversely, urinary obstruction and end-stage renal disease cases were relatively higher in dogs than in human populations.

Keyword

dog diseases; renal diseases

MeSH Terms

Animals
Dog Diseases/*pathology
Dogs
Female
Humans
Kidney/*pathology
Kidney Diseases/pathology/*veterinary
Male
Republic of Korea
Retrospective Studies

Figure

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  Fig. 1 Canine glomerulonephritis (GN). (A) Acute proliferative GN. The glomerulus was congested with high cellularity. Inset: Glomerulus of normal kidney showing normal celluarity and absence of congestion. H&E stain. Scale bar = 105 µm. (B) Membranous GN. Irregularly-thickened glomerular basement membranes were visible with decreased amounts of blood in the glomeruli without glomerular congestion. Periodic acid Schiff (PAS) stain. Scale bar = 105 µm. (C) Membranoproliferative GN. Both proliferative and membranous changes were observed. Irregularly-thickened glomerular basement membranes with endocapillary hypercellularity were observed. Inset: Detail of thickened glomerular basement membranes. PAS stain. Scale bar = 105 µm. (D) Focal segmental glomerulosclerosis was characterized by sclerosis of a few of glomeruli with involvement of only a portion of the capillary tuft. PAS stain. Scale bar = 210 µm. (E) Infiltrated amyloid was identified in the renal glomerulus. Congo Red stain. Scale bar = 50 µm. (F) Weak and diffuse labeling with IgA antibody was identified in the renal glomerulus in IgA nephropathy case. IHC, with Harris haematoxylin counterstain. Scale bar = 50 µm.

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  Fig. 2 Canine IgA nephropathy. The basement membrane showed diffuse and intense granular deposits. IF using fluorescein isothiocyanate-anti-goat IgG. Scale bar = 35 µm.

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  Fig. 3 Canine tubulointerstitial nephritis. (A) Chronic interstitial nephritis. Prominent interstitial fibrosis and tubular atrophy without severe glomerular changes was evident. Immunocyte interstitial infiltrates contained lymphocytes, plasma cells, and macrophages. (B) Pyelonephritis. Medullary necrosis and immunocyte infiltration including neutrophils were observed. H&E stain. Scale bar = 70 µm.

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  Fig. 4 Canine melamine-associated renal failure (MARF). (A) Acute MARF. Severe distal tubular necrosis with intratubular crystals and no significant interstitial inflammation or fibrosis characteristic of MARF. H&E stain. (B) Chronic MARF. Intratubular crystals appeared in the distal tubules. H&E stain. (C) Chronic MARF. Chronic MARF demonstrated histopathologic changes of severe renal interstitial fibrosis in the serial section. Masson's trichrome stain. Scale bar = 210 µm.

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  Fig. 5 Canine renal cell carcinoma. (A) Papillary cell-type renal cell carcinoma. Papillae and tubules were lined by a single layer of epithelium. H&E stain. Scale bar = 210 µm. (B) Tubular-type renal cell carcinoma. Well-differentiated tubular cells consisting of solid tumor tissues. The cytoplasm was lightly eosinophilic, and many mitotic figures were observed. H&E stain. Scale bar = 105 µm. (C) Clear cell-type renal cell carcinoma. Large cells with abundant, light to clear cytoplasm, and uniform central nuclei characteristic of this tumor type. H&E stain. Scale bar = 105 µm.

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  Fig. 6 Canine end-stage renal disease (ESRD). (A) ESRD samples demonstrated extensive areas of fibrosis; deposition of extracellular matrix, glomerular, and tubular (proximal and distal) destruction; and severe renal calcification. H&E stain. (B) Stained blue areas represented renal cortical fibrosis of canine ESRD kidneys with visible infiltrating immunocytes. Masson's trichrome stain. Scale bars = 140 µm.

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