J Korean Med Sci.  1998 Apr;13(2):159-164. 10.3346/jkms.1998.13.2.159.

Detection of MYC gene amplification in malignant lymphomas

• Park CK
• Lee CG
• Lee JD

• KMID: 1064963
• DOI: http://doi.org/10.3346/jkms.1998.13.2.159

Abstract

The role of oncogene or tumor supressor gene in Epstein-Barr virus (EBV) associated malignant lymphomas (MLs) is poorly understood. We examined 36 MLs (21 EBV positive and 15 EBV negative) and 6 reactive hyperplasias for the presence of myc gene amplification. Polymerase chain reaction (PCR) technology was used to examine the state of amplification of the proto-oncogene c-myc in formalin-fixed paraffin-embedded tissues. Variable degrees of myc gene amplification were detected in reactive hyperplasias and MLs. However, significant increase of c-myc copy numbers above 3 times were only found in 12 out of 31 non-Hodgkin's MLs (38.7%), in which 6 cases were EBV positive and 6 cases were EBV negative. In conclusion, myc gene amplification appears to play a part in MLs but no correlation was found between EBV infection and myc gene amplification.

MeSH Terms

DNA, Viral/analysis
Gene Amplification*
Giant Lymph Node Hyperplasia/genetics
Herpesvirus 4, Human/isolation & purification
Herpesvirus 4, Human/genetics
Hodgkin Disease/virology
Hodgkin Disease/genetics*
Human
Hyperplasia/genetics
Lymphoma, Non-Hodgkin/virology
Lymphoma, Non-Hodgkin/genetics*
Polymerase Chain Reaction
Proto-Oncogene Proteins c-myc/genetics*