J Korean Med Sci.  1999 Feb;14(1):15-20. 10.3346/jkms.1999.14.1.15.

Rapid and easy detection of Helicobacter pylori by in situ hybridization

• Park CS
• Kim J

• KMID: 1062191
• DOI: http://doi.org/10.3346/jkms.1999.14.1.15

Abstract

Various in situ hybridization (ISH) methods have been used to identify Helicobacter pylori, a causative organism responsible for chronic gastritis and peptic ulcer disease, but they were hard to perform and time consuming. To detect H. pylori in a rapid and easily reproducible way, we developed synthetic biotinylated oligonucleotide probes which complement rRNA of H. pylori. Formalin-fixed and paraffin-embedded tissues from 50 gastric biopsy specimens were examined. Using a serologic test and histochemical stain (Warthin-Starry silver stain and/or Giemsa stain) as a standard, 40 of them were confirmed to be H. pylori-positive. Our ISH was quickly carried out within one hr and results were compared with those obtained from immunohistochemical stain. The ISH produced a positive reaction in 38 of 40 cases (95%). All H. pylori-negative cases failed to demonstrate a positive signal. The ISH has a sensitivity comparable to those of conventional histochemical and immunohistochemical stain, and has high specificity. In conclusion, ISH with a biotinylated oligonucleotide probe provides a useful diagnostic method for detecting H. pylori effectively in routinely processed tissue sections.

Keyword

Helicobacter pylori; In situ hybridization; RNA, ribosomal; Biopsy

MeSH Terms

Helicobacter Infections/pathology
Helicobacter Infections/microbiology*
Helicobacter pylori/isolation & purification*
Helicobacter pylori/genetics
Human
In Situ Hybridization/methods*
Oligonucleotide Probes
RNA, Bacterial/analysis
RNA, Ribosomal, 16S/analysis
Sensitivity and Specificity
Silver Staining/methods
Time Factors