Korean J Parasitol.  2004 Jun;42(2):85-89. 10.3347/kjp.2004.42.2.85.

Genotype and animal infectivity of a human isolate of Cryptosporidium parvum in the Republic of Korea

• Guk SM
• Yong TS
• Park SJ
• Park JH
• Chai JY

• KMID: 1051425
• DOI: http://doi.org/10.3347/kjp.2004.42.2.85

Abstract

Cryptosporidium parvum oocysts were isolated from a child suffering from acute gastroenteritis and successfully passaged in a calf and mice (designated hereafter SNU-H1) in the Republic of Korea; its molecular genotype has been analyzed. The GAG microsatellite region was amplified by a polymerase chain reaction (PCR), with a 238 base pair product, which is commonly displayed in C. parvum. The isolate was shown to be a mixture of the genotypes 1 (anthroponotic) and 2 (zoonotic). To study its infectivity in animals, 2 calves and 3 strains of mice were infected with the SNU-H1; in these animals, the propagation of both genotypes was successful. In immunosuppressed (ImSP) BALB/c and C57BL/6 mice the number of oocysts decreased after day 10 post-infection (PI) ; but in ImSP ICR mice, they remained constant until day 27 PI. The results show that both the C. parvum genotypes 1 and 2 can be propagated in calves and ImSP mice.

Keyword

Cryptosporidium parvum; oocyst; human; calf; mouse; genotype

MeSH Terms

Animals
Cattle
Child
Cryptosporidiosis/microbiology
Cryptosporidium parvum/*genetics/immunology
Diarrhea/parasitology
Feces/parasitology
Genotype
Human
Korea
Male
Mice
Mice, Inbred BALB C
Mice, Inbred C57BL
Mice, Inbred ICR
Oocysts
Polymerase Chain Reaction
Support, Non-U.S. Gov't
Zoonoses/parasitology