Korean J Parasitol.  2000 Mar;38(1):45-48. 10.3347/kjp.2000.38.1.45.

Isozyme electrophoresis patterns of the liver fluke, Clonorchis sinensis from Kimhae, Korea and from Shenyang, China

• Park GM
• Yong TS
• Im K
• Lee KJ

• KMID: 1040998
• DOI: http://doi.org/10.3347/kjp.2000.38.1.45

Abstract

An enzyme analysis of the liver fluke, Clonorchis sinensis from Kimhae, Korea and from Shenyang, China was conducted using a horizontal starch gel electrophoresis in order to elucidate their genetic relationships. A total of eight enzymes was employed from two different kinds of buffer systems. Two loci from each enzyme of aconitase and esterase (alpha-Na and beta-Na); and only one locus each from six enzymes, glucose-6-phosphate dehydrogenase (G6PD), alpha-glycerophosphate dehydrogenase (GPD), 3-hydroxybutyrate dehydrogenase (HBDH), malate dehydrogenase (MDH), phosphoglucose isomerase (PGI), and phosphoglucomutase (PGM) were detected. Most of loci in two populations of C. sinensis showed homozygous monomorphic banding patterns and one of them, GPD was specific as genetic markers between two different populations. However, esterase (alpha-Na), GPD, HBDH and PGI loci showed polymorphic banding patterns. Two populations of C. sinensis were more closely clustered within the range of genetic identity value of 0.998-1.0. In summarizing the above results, two populations of C. sinensis employed in this study showed mostly monomorphic enzyme protein banding patterns, and genetic differences specific between two populations.

MeSH Terms

Aconitate Hydratase/isolation & purification
Aconitate Hydratase/genetics
Alleles
Animal
China
Clonorchis sinensis/genetics
Clonorchis sinensis/enzymology*
Electrophoresis, Starch Gel
Esterases/isolation & purification
Esterases/genetics
Glucosephosphate Dehydrogenase/isolation & purification
Glucosephosphate Dehydrogenase/genetics
Isoenzymes/isolation & purification
Isoenzymes/genetics
Korea