Exp Mol Med.  2005 Feb;37(1):1-10.

Proteomic applications of surface plasmon resonance biosensors: analysis of protein arrays

• Yuk JS
• Ha KS

Abstract

Proteomics is one of the most important issues in the post-genomic area, because it can greatly contribute to identifying protein biomarkers for disease diagnosis and drug screening. Protein array is a key technology for proteome researches and has been analyzed by various methods including fluorescence, mass spectrometry, atomic force microscopy and surface plasmon resonance (SPR). SPR biosensor is a promising technology in proteomics, since it has various advantages including real-time measurement of biomolecular interactions without labeling and the simple optical system for the device. SPR biosensors have a strong potential for analyzing proteomes by SPR imaging and SPR spectroscopic imaging, even though the challenge is to produce proteins on a proteomic scale.

Keyword

biosensing techniques; microscopy; atomic force; protein array analysis; proteome; proteomics; surface plasmon resonance

MeSH Terms

Animals
*Biosensing Techniques
Humans
Protein Array Analysis
*Proteomics
Research Support, Non-U.S. Gov't
*Surface Plasmon Resonance/instrumentation/methods