Exp Mol Med.
2011 Jul;43(7):427-435. 10.3858/emm.2011.43.7.047.
Discovery of the serum biomarker proteins in severe preeclampsia by proteomic analysis
- Affiliations
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- 1Department of Molecular Biotechnology, Institute of Biomedical Science and Technology, Konkuk University, Seoul 143-701, Korea. kpkim@konkuk.ac.kr
- 2Department of Obstetrics and Gynecology, Kangnam CHA Hospital, CHA University, Seoul 135-080, Korea.
- 3Department of Obstetrics and Gynecology, Inje University, Pusan Paik Hospital, Busan 614-735, Korea.
- 4Division of Mass Spectrometry Research, Korea Basic Science Institute, Ochang 363-883, Korea.
- KMID: 1015462
- DOI: http://doi.org/10.3858/emm.2011.43.7.047
Abstract
- Preeclapsia (PE) is a severe disorder that occurs during pregnancy, leading to maternal and fetal morbidity and mortality. PE affects about 3-8% of all pregnancies. In this study, we conducted liquid chromatographymass spectrometry/mass spectrometry (LC-MS/MS) to analyze serum samples depleted of the six most abundant proteins from normal and PE-affected pregnancies to profile serum proteins. A total of 237 proteins were confidently identified with < 1% false discovery rate from the two groups of duplicate analysis. The expression levels of those identified proteins were compared semiquantitatively by spectral counting. To further validate the candidate proteins with a quantitative mass spectrometric method, selective reaction monitoring (SRM) and enzyme linked immune assay (ELISA) of serum samples collected from pregnant women with severe PE (n = 8) or normal pregnant women (n = 5) was conducted. alpha2-HS-glycoprotein (AHSG), retinol binding protein 4 (RBP4) and alpha-1-microglobulin/bikunin (AMBP) and Insulin like growth factor binding protein, acid labile subunit (IGFBP-ALS) were confirmed to be differentially expressed in PE using SRM (P < 0.05). Among these proteins, AHSG was verified by ELISA and showed a statistically significant increase in PE samples when compared to controls.
Keyword
MeSH Terms
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Adult
Alpha-Globulins/metabolism
Amino Acid Sequence
Biological Markers/blood
Blood Proteins/*analysis
Case-Control Studies
Female
Humans
Insulin-Like Growth Factor Binding Proteins/blood
Molecular Sequence Data
Pre-Eclampsia/*blood/diagnosis
Pregnancy
Proteome/*analysis
Retinol-Binding Proteins, Plasma/metabolism
alpha-2-HS-Glycoprotein/metabolism
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