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Differential Effects of Two Widely Used Solvents, DMSO and Ethanol, on the Growth and Recovery of Trypanosoma cruzi Epimastigotes in Culture

Cevallos A, Herrera J, López-Villaseñor I, Hernández R

Trypanosoma cruzi is the etiological agent of Chagas disease. Epimastigote forms of T. cruzi can be readily cultured in axenic conditions. Ethanol and dimethyl sulfoxide (DMSO) are commonly used solvents...
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Trypanosome Glycosylphosphatidylinositol Biosynthesis

Hong Y, Kinoshita

Trypanosoma brucei, a protozoan parasite, causes sleeping sickness in humans and Nagana disease in domestic animals in central Africa. The trypanosome surface is extensively covered by glycosylphosphatidylinositol (GPI)-anchored proteins known...
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Towards developing a diagnostic regimen for the treatment follow-up of Trypanosoma brucei gambiense

Mbati PA, Hirumi K, Inoue N, Situakibanza NH, Hirumi H

BALB/c mice infected with a high virulent strain of Trypanosoma brucei gambiense IL3707 were treated intraperitoneally (ip) with either Melarsoprol (Mel-B) or PSG(+) buffer as controls. The mice were subsequently...
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Epizootic Infection by Trypanosoma vivax in Cattle from the State of Minas Gerais, Brazil

de Oliveira Reis M, Souza FR, Albuquerque AS, Monteiro F, dos Santos Oliveira LF, Raymundo D, Wouters F, Wouters AT, Peconick A, Varaschin

Trypanosomiasis is caused by a pathogenic protozoan of the genus Trypanosoma, being Trypanosoma vivax the most important agent for cattle. The aim of the present study was to demonstrate the...
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Quantification of Immunoglobulin G against Trypanosoma cruzi in Individuals with Chronic Chagas Disease Treated with Nifurtimox and Evaluated in Prolonged Follow-Up

Muñoz G, Vergara C, Martínez , Apt W, Zulantay I

In the indeterminate chronic period of Chagas disease (ChD) the treatment has not been conclusive, because the serological negativization requires many years. This study aims to evaluate the efficacy of...
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Detection of Human Anti-Trypanosoma cruzi Antibody with Recombinant Fragmented Ribosomal P Protein

Kim YH, Yang Z, Lee J, Ahn HJ, Chong CK, Maricondi , Dias RF, Nam HW

Chagas disease is caused by the protozoan parasite Trypanosoma cruzi, and is endemic in many Latin American countries. Diagnosis is based on serologic testing and the WHO recommends two or...
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Trypanosoma brucei Infection in Asymptomatic Greater Kudus (Tragelaphus strepsiceros) on a Game Ranch in Zambia

Munang'andu HM, Siamudaala V, Munyeme M, Nambota A, Mutoloki S, Matandiko

Trypomastogotes of Trypanosoma brucei were detected from 4 asymptomatic kudus (Tragelaphus strepsiceros) on a game ranch located approximately 45 km north east of Lusaka, Zambia. Blood smears examined from 14...
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Prehistoric Pathoecology as Represented by Parasites of a Mummy from the Peruaçu Valley, Brazil

Reinhard K, Araújo A

Paleopathologists have begun exploring the pathoecology of parasitic diseases in relation to diet and environment. We are summarizing the parasitological findings from a mummy in the site of Lapa do...
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Oxidative Stress in the Heart of Rats Infected with Trypanosoma evansi

Baldissera MD, Souza CD, Bertoncheli CM, da Silveira KL, Grando TH, Porto BC, Leal DB, Silva AS, Mendes RE, Stefani LM, Monteiro S

This study was conducted to investigate the occurrence of oxidative stress in the heart tissue of rats infected with Trypanosoma evansi. Rats were divided into 2 groups (A and B)...
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In Vitro Trypanocidal Activity of Macela (Achyrocline satureioides) Extracts against Trypanosoma evansi

Baldissera MD, Oliveira C, Zimmermann CEP, Boligon AA, Athayde ML, Bolzan LP, Vaucher RD, Santurio J, Sagrillo M, da Silva A, Monteiro S

  • KMID: 1792188
  • Korean J Parasitol.
  • 2014 Jun;52(3):311-315.
The aim of this study was to verify the trypanocidal effectiveness of aqueous, methanolic, and ethanolic extracts of Achyrocline satureioides against Trypanosoma evansi in vitro. A. satureioides extracts, known as...
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Molecular Characterization of Trypanosoma cruzi Tc8.2 Gene Indicates Two Differential Locations for the Encoded Protein in Epimastigote and Trypomastigote Forms

Kian D, Lancheros , Damiani IA, Fernandes , Pinge-Filho P, dos Santos MRM, da Silveira JF, Nakamura C, da Silva J, Yamada-Ogatta SF, Yamauchi L

This report describes the molecular characterization of the Tc8.2 gene of Trypanosoma cruzi. Both the Tc8.2 gene and its encoded protein were analyzed by bioinformatics, while Northern blot and RT-PCR...
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Susceptibility of Mice to Trypanosoma evansi Treated with Human Plasma Containing Different Concentrations of Apolipoprotein L-1

Da Silva A, Fanfa VR, Otto MA, Gressler L, Tavares KC, Lazzarotto CR, Tonin AA, Miletti LC, Duarte MM, Monteiro S

The aim of this study was to test the susceptibility of mice to Trypanosoma evansi treated with human plasma containing different concentrations of apolipoprotein L-1 (APOL1). For this experiment, a...
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Evaluation of SD BIOLINE Chagas Ab Rapid Kit

Ji MJ, Noh JS, Cho BK, Cho YS, Kim SJ, Yoon BS

BACKGROUND: Chagas' disease is caused by Trypanosoma cruzi, a protozoan parasite, which is transmitted by blood-sucking bugs or through blood transfusion or organ transplantation. It is endemic in Central and...
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Assessing the Archaeoparasitological Potential of Quids As a Source Material for Immunodiagnostic Analyses

Morrow JJ, Reinhard K

In the present study, quids from La Cueva de los Muertos Chiquitos (CMC) were subjected to ELISA tests for 2 protozoan parasites, Toxoplasma gondii (n=45) and Trypanosoma cruzi (n=43). The...
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Inhibition of ER Stress by 2-Aminopurine Treatment Modulates Cardiomyopathy in a Murine Chronic Chagas Disease Model

Ayyappan J, lizardo K, Wang S, Yurkow E, Nagajyothi JF

Trypanosoma cruzi infection results in debilitating cardiomyopathy, which is a major cause of mortality and morbidity in the endemic regions of Chagas disease (CD). The pathogenesis of Chagasic cardiomyopathy (CCM)...
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Endoparasites of Small Mammals in Edo State, Nigeria: Public Health Implications

Isaac C, Igbinosa B, Ohiolei JA, Osimen CE

Some small mammals occur as household pests and harbour a number of parasites that could be of public health importance. This study profiled the helminth and protozoan parasites in trapped...
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