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Inadvertent Staining of the Vitreous with Trypan Blue During Cataract Surgery

Lee MY, Lee HS, Joo CK

PURPOSE: We report a case of inadvertent staining of the vitreous with Trypan Blue during cataract surgery. CASE SUMMARY: We performed cataract surgery in a 44-year-old male patient on an eye...
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Trypan Blue-Assisted Measurements of Anatomical Positions of the Superior Rectus Muscle and Superior Oblique Muscle in Enucleated Eyes

Shin YT, Chun BY

  • KMID: 2338274
  • J Korean Ophthalmol Soc.
  • 2012 Oct;53(10):1488-1492.
PURPOSE: To recognize the anatomical positions of the superior oblique muscle in enucleated eyes using trypan blue. METHODS: Twenty-two surgically-enucleated eyes of 11 bodies were studied. The shortest distance from the...
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Effect of Trypan Blue on the Survival of Cultured Trabecular Meshwork Cells

Kang HR, Hwang SJ, Kim JW

PURPOSE: To evaluate the effects of trypan blue (TB) on the survival of cultured human trabecular meshwork cells (HTMCs). METHODS: Primarily cultured HTMCs were exposed to 0.05, 0.10 or 0.50% TB...
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Assessment of Cell Viability in Umbilical Cord Blood by Using 7-AAD/annexin V Dual Staining

Kim KM, Huh JY, Kang MS, Jung SH

  • KMID: 2312235
  • Lab Med Online.
  • 2014 Jan;4(1):1-7.
BACKGROUND: The quality of cord blood largely depends on cell viability. Viability assessments using trypan blue or 7-aminoactinomycin (7-AAD) staining, which are commonly used methods, may not reflect early apoptosis...
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Comparison of three supravital stains and acridine orange test in examination of human semen

Oum KB, Shin AJ, Ko JJ, Suh DW, Cha KY

  • KMID: 2136846
  • J Korean Androl Soc.
  • 1993 Jun;11(1):7-12.
Twelve human semen specimens were used to compare the reliability of the eosin, trypan blue, and hoechst supravital tests. The acridine orange test was also carried out to investigate whether...
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Chromosome Aberrations in Malformed Rat Fetuses Induced with Trypan Blue

Lee MS, Wang CS

  • KMID: 1677715
  • Korean J Urol.
  • 1976 Sep;17(3):153-161.
It is now well known that several human congenital syndromes are accompanied by chromosomal aberrations. Chromosomal aberrations can also be induced by several teratogenic agents in various species of animals...
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Assessment of Cell Viability in Umbilical Cord Blood before Cryopreservation

Yi DY, Huh JY, Kang MS

  • KMID: 2090012
  • Korean J Blood Transfus.
  • 2010 Aug;21(2):140-147.
BACKGROUND: The viability of cord blood is an important measure of product quality. Trypan blue (TB) stain is the most commonly and conveniently used method to measure the viability of...
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Stimulatory Effects of Ginsan on the Proliferation and Viability of Mouse Spleen Cells

Ko EJ, Joo HG

Ginsan is an acidic polysaccharide purified from Panax ginseng, a famous oriental herb. Although a variety of biological activities of ginsan have been studied, the effects of ginsan on spleen...
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Effects of Topically Applied Na-Hya luronan on Stromal and Endothelial Healing in Experimental Corneal Alkali Wounds

Park YK, Chung JH

  • KMID: 1951312
  • J Korean Ophthalmol Soc.
  • 2000 Aug;41(8):1631-1637.
The effects of topical sodium-hyaluronan on the stromal and endothelial healing were examined in the repair process of experimental corneal alkali wounds. Corneal alkali wounds were produced in one eye...
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In vitro Effect of Sophora flavescens on the Ultrastructure of Trichomonas vaginalis Donne

Choi WG, Cho YJ, Chu JP

  • KMID: 2082960
  • Korean J Infect Dis.
  • 2002 Aug;34(4):248-254.
BACKGROUND: Trichomonas vaginalis is a pathogenic protozoa infecting human genitourinary tract. Metronidazole is currently the drug of choice to treat T. vaginalis infection. However, because of the side effects and...
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The Effect of Steroid on the Viability of the Endothelial Cell of Stored Cornea

Hyun PM, Lee JH

  • KMID: 2042559
  • J Korean Ophthalmol Soc.
  • 1986 Dec;27(6):1001-1007.
Autolysis, which is brought about by the release of lysosomal hydrolytic enzymes, has been prevented in cornea with the use of lysosomal membrane stabilizers, such as corticosteroid. So, we performed...
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Investigating the Sonodynamic-Radiosensitivity Effect of Gold Nanoparticles on HeLa Cervical Cancer Cells

Shanei A, Akbari-Zadeh H

BACKGROUND: In this article, we estimated the combined effect of radiotherapy (RT) with ultrasound (US) wave and the ability of gold nanoparticles (GNPs) to improve their combined therapeutic effects. METHODS: At...
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Optimal Media Conditions for the Detection of Extracellular Cellulase Activity in Ganoderma neo-japonicum

Jo WS, Park HN, Cho DH, Yoo YB, Park SC

  • KMID: 2312629
  • Mycobiology.
  • 2011 Jun;39(2):129-132.
To determine the optimal media conditions for the detection of the extracellular cellulase activity in Ganoderma neo-japonicum, we varied three media conditions: dye reagent, pH, and temperature. We evaluated the...
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Optimal Medium Conditions for the Detection of Cellulolytic Activity in Ganoderma lucidum

Jo WS, Bae SH, Cho DH, Park SD, Yoo YB, Park SC

To determine the optimal medium conditions for the detection of the cellulolytic activity in Ganoderma lucidum, we varied three media conditions: dye reagent, pH, and temperature. First, we evaluated the...
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Cytotoxicity of Vibrio vulnificus cytolysin on pulmonary endothelial cells

Kim JS

  • KMID: 2045607
  • Exp Mol Med.
  • 1997 Jun;29(2):117-121.
The cytolysin produced by V. vulnificus has been known to cause lethality by increasing pulmonary vascular permeability in mice. In the present study, its cytotoxic mechanism on CPAE cell, a...
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Difference in Viability of CD34+Cells in Cryopreserved Cord Blood According to Evaluation Methods

Ahn MS, Eom YW, Park JS, Choi JH, Kang SY, Lee HW, Yang MS, Kim HE, Jang IK, Lee JE, Kim YJ, Kim HC, Jeong SH

BACKGROUND: On performing umbilical cord blood (UCB) transplantation, faster engraftment may lead better clinical outcome. Because transplanted viable cell count in UCB is related to the engraftment, accurate evaluation of...
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Effects of Topically Applied 0.1%Dexamethasone on Endothelial Healing and Aqueous Composition Following Experimental Corneal Alkali Wounds

Park YK, Chung JH

  • KMID: 2124720
  • J Korean Ophthalmol Soc.
  • 2000 May;41(5):1033-1039.
The effects of topical dexamethasone on the endothelial healing and the change of aqueous composition were examined in the repair process of experimental corneal alkali wounds. Corneal alkali wounds were...
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Effects of the Peroxisome Proliferator-Activated Receptor Ligand Troglitazone in Osteosarcoma Cell Lines

Kim YJ, Kim TK, Park JY, Kim HJ, Lee JW

  • KMID: 2106194
  • J Korean Orthop Assoc.
  • 2005 Sep;40(5):591-597.
PURPOSE: We wanted to investigate the effects of Troglitazone (TRO) on the cellular growth in two human osteosarcoma cell lines (U2OS and HOS). MATERIALS AND METHODS: Cell viability was assessed...
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Effects of Carboplatin in Combination with Hyperthermia on Cell Death in Retinoblastoma Cell Line

Seo SG, Park HS, Yun IH

  • KMID: 2125263
  • J Korean Ophthalmol Soc.
  • 2002 Nov;43(11):2310-2322.
PURPOSE: The effect of environmental acidity on the induction of apoptosis by heat and carboplatin alone or combined was investigated. METHODS: Human retinoblastoma Y79 and WERI cells were treated with hyperthermia...
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Immunostimulatory Effects of beta-glucan Purified from Paenibacillus polymyxa JB115 on Mouse Splenocytes

Kim JM, Joo HG

We investigated the effects of beta-glucan purified from Paenibacillus polymyxa JB115 on the viability and proliferation of splenocytes. Splenocytes play a critical role in host immunity. MTT assays and trypan...
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