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Heterologous Expression of Endo-1,4-beta-xylanaseA from Phanerochaete chrysosporium in Pichia pastoris

Huy ND, Thiyagarajan S, Son YL, Park SM

  • KMID: 2312627
  • Mycobiology.
  • 2011 Jun;39(2):121-124.
The cDNA of endo-1,4-beta-xylanaseA, isolated from Phaenerocheate chrysosporium was expressed in Pichia pastoris. Using either the intrinsic leader peptide of XynA or the alpha-factor signal peptide of Saccharomyces cerevisiae, xylanaseA...
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Two Cases of Pichia Ohmeri Fungemia

Joo SW, Yoon WS, Kook H, Cho CY, Choi YY, Hwang TJ, Shin JH

  • KMID: 2278237
  • Korean J Pediatr Hematol Oncol.
  • 2001 Apr;8(1):137-141.
Pichia ohmeri is an yeast-like fungus used in the food industry for fermentation. This organism has been implicated in human disease only in a few case reports. We describe herewith...
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A Case of Pichia anomala Detected Incidentally in Peripheral Blood Smear

Heo WB, Kim YK, Lee NY, Lee WK

  • KMID: 2224699
  • Korean J Clin Microbiol.
  • 2005 Apr;8(1):94-98.
Nosocomial opportunistic infections including fungal infections continue to increase with a longer survival of immunocompromised patients. Disseminated candidiasis is the most common nosocomial fungal infection and the frequency of isolation...
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Physiological Functionality and Enzyme Activity of Biomass from Pichia anomala Grown on Ginseng-Steaming Effluent

Kim NM, So SH, Lee SG, Song JE, Seo DS, Lee JS

A novel biomass was prepared from Pichia anomala KCCM 11473, which grew well in ginseng-steaming effluent (GSE), and its physiological functionalities and enzyme activities were determined. When the strain was...
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Production and Its Anti-hyperglycemic Effects of γ-Aminobutyric Acid from the Wild Yeast Strain Pichia silvicola UL6-1 and Sporobolomyces carnicolor 402-JB-1

Han SM, Lee JS

This study was done to produce γ-aminobutyric acid (GABA) from wild yeast as well as investigate its anti-hyperglycemic effects. Among ten GABA-producing yeast strains, Pichia silvicola UL6-1 and Sporobolomyces carnicolor...
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A Case of Malignant External Otitis with Pichia ohmeri

Son JS, Shin SY, Min TH, Chang HH, Jung SI, Oh WS, Kim SM, Peck KR, Song JH

  • KMID: 2248676
  • Korean J Infect Dis.
  • 2002 Oct;34(5):349-353.
Pichia ohmeri is a rare fungal pathogen for human infection. To date, only 5 cases of P. ohmeri infection have been reported including fungemia, disseminated infection and peritonitis. We report...
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Effect of Different Pretreatment Methods on the Bioconversion of Rice Bran into Ethanol

Eyini M, Rajapandy , Parani K, Lee MW

  • KMID: 2312444
  • Mycobiology.
  • 2004 Dec;32(4):170-172.
The efficiency of acid, enzyme and microbial pretreatment of rice bran was compared based on the content of cellulose, hemicellulose, reducing sugars and xylose in the substrate. An isolate of...
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Heterologous Expression of Phanerochaete chrysoporium Glyoxal Oxidase and its Application for the Coupled Reaction with Manganese Peroxidase to Decolorize Malachite Green

Son YL, Kim HY, Thiyagarajan S, Xu JJ, Park SM

cDNA of the glx1 gene encoding glyoxal oxidase (GLX) from Phanerochaete chrysosporium was isolated and expressed in Pichia pastoris. The recombinant GLX (rGLX) produces H2O2 over 7.0 nmol/min/mL using methyl...
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A Case of Pichia ohmeri Fungemia Related to the Central Venous Catheter

Lee JH, Kim CK, Lee HS, Kim JS, Kim MJ, Chung OY, Kim JR, So MJ, Na BJ, Cho YK, Lee KR, Lee MH, Rho YH

  • KMID: 2248718
  • Korean J Infect Dis.
  • 2000 Jun;32(3):252-255.
Pichia ohmeri is a very rare pathogen of human infection. To date, two cases of P. ohmeri infection were reported worldwide. We have experienced a case of catheter-related P. ohmeri...
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Microbiological Characteristics of Wild Yeast Strain Pichia anomala Y197-13 for Brewing Makgeolli

Kim HR, Kim JH, Bai DH, Ahn BH

Makgeolli is a traditional cloudy-white Korean rice wine with an alcohol content of 6~7%. The present study investigated the morphological characteristics, carbon-utilizing ability, fatty acid composition, alcohol resistance, glucose tolerance,...
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Estimation of Cell Concentration by Light Transmitter During the Culture of Methylotrophic Yeast Pichia pastoris

Choi DB, Park EY, Lee YB, Na YH, Lim CK

  • KMID: 2312418
  • Mycobiology.
  • 2003 Dec;31(4):226-228.
The multiple correlation coefficient between the values determined by dry weight and those determined by fluorometer was observed with r = 0.96 and the standard error of calibration was 0.034....
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Characterization of two antimicrobial peptides identified from a random peptide library and expressed in the methylotrophic yeast pichia pastoris

Jeon IS, Song KD, Oh JD, Choi JK

  • KMID: 1703518
  • J Biomed Res.
  • 2013 Dec;14(4):235-239.
Antimicrobial peptides (AMPs) are an important component of innate defense mechanisms with broad-spectrum activities against various pathogenic microorganisms, including Gram-positive and Gram-negative bacteria, fungi, and viruses. Antibiotic resistance has become...
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Differential Expression of Laccase Genes in Pleurotus ostreatus and Biochemical Characterization of Laccase Isozymes Produced in Pichia pastoris

Park M, Kim M, Kim S, Ha B, Ro HS

In this study, transcriptome analysis of twelve laccase genes in Pleurotus ostreatus revealed that their expression was differentially regulated at different developmental stages. Lacc5 and Lacc12 were specifically expressed in...
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Successful Treatment of Catheter Related Blood Stream Infection By Millerozyma farinosa with Micafungin: A Case Report

Hong SI, Suh YS, Kim HO, Bae IG, Shin JH, Cho OH

Millerozyma farinosa (formerly Pichia farinosa) is halotolerant yeast mainly found in food and ubiquitous in the environment. It was a rare yeast pathogen, but it has recently emerged as a...
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Successful Treatment of Fungemia Caused by Cyberlindnera fabianii with Anidulafungin: A Case Report

Lee JI, Yu S, Park JS, Joo EJ, Shin JH, Kwon MJ

Cyberlindnera fabianii (previously known as Hansenula fabianii, Pichia fabianii, and Lindnera fabianii) is a yeast species that forms a biofilm, allowing it to resist azole drugs. In this study, we...
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Kodamaea ohmeri Fungemia in a Patient with Carcinoma of the Ampulla of Vater

Son BR, Shin KS

Kodamaea ohmeri, previously known as Pichia ohmeri or Yamadazyma ohmeri, is an ascosporogenous yeast that belong to the Saccharomycetaceae family, and it is a rare yeast-form fungus recently identified as...
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Screening Wild Yeast Strains for Alcohol Fermentation from Various Fruits

Lee YJ, Choi YR, Lee SY, Park JT, Shim JH, Park KH, Kim JW

  • KMID: 2312608
  • Mycobiology.
  • 2011 Mar;39(1):33-39.
Wild yeasts on the surface of various fruits including grapes were surveyed to obtain yeast strains suitable for fermenting a novel wine with higher alcohol content and supplemented with rice...
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Comparison of Microbial Diversity of Korean Commercial Makgeolli Showing High beta-Glucan Content and High Antihypertensive Activity, Respectively

Min JH, Kim YH, Kim JH, Choi SY, Lee JS, Kim HK

We measured physiological functionalities, including antihypertensive angiotensin I-converting enzyme inhibitory activity and immun-stimulating beta-glucan content for sixty kinds of Makgeolli that is commercially available from the market. As a result,...
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Profiles of IgE Sensitization to Der f 1, Der f 2, Der f 6, Der f 8, Der f 10, and Der f 20 in Korean House Dust Mite Allergy Patients

Jeong KY, Lee JY, Son M, Yi MH, Yong TS, Shin JU, Lee KH, Kim YJ, Park KH, Park HJ, Lee JH, Park JW

PURPOSE: Measurement of IgE specific to purified house dust mite (HDM) allergens may improve allergy diagnosis. This study aimed to investigate the sensitization profiles of Korean HDM allergic subjects suffering...
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Effects of Ser47-Point Mutation on Conformation Structure and Allergenicity of the Allergen of Der p 2, a Major House Dust Mite Allergen

Kulwanich , Thanyaratsrisakul S, Jirapongsananuruk O, Hales BJ, Thomas WR, Piboonpocanun S

PURPOSE: Hypoallergenic recombinant Der p 2 has been produced by various genetic manipulations, but mutation of a naturally polymorphic amino acid residue known to affect IgE binding has not been...
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