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Antibody-dependent rat macrophage-mediated damage to the excysted metacercariae of Paragonimus westermani in vitro

Chung PR, Chang JK, Soh CT

An in vitro immune effector mechanism against the target excysted metacercariae of Paragonimus westermani was demonstrated in the rat system. Peritoneal exudate cells, mainly macrophages from normal rats, showed adherence...
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Tissue origin of soluble component proteins in saline extract of adult Paragonimus westermani

Kong Y, Park CY, Kang SY, Cho SY

Tissue origin of individual component proteins in crude extract of adult Paragonimus westermani was investigated. Major soluble component proteins were separated by disc-PAGE in 8% slab gel. By predefined Rf...
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Antigenic localities in the tissues of Paragonimus westermani by developmental stages using immunogold labeling method

Rim HJ, Kim SJ, Sun IJ, Lee JS

In order to observe the antigenic localization in the tissues of Paragonimus westermani of developmental stages, immunogold labeling method was applied using serum of the cats which were infected with...
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Protein composition and antigenicity of the tegument from Paragonimus westermani

Kim SI, Cho SY

To ascertain that tegument of Paragonimus westermani has specific antigenic proteins, the tegumental fraction was isolated from 10-month-old worms by 0.1% digitonin solution, and subjected to SDS-PAGE and immunoblot. Component...
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Antigenic localities in the tissues of the young adult worm of Paragonimus westermani using immunogold labeling method

Kwon OS, Lee JS, Rim HJ, Kim SJ

In order to observe the antigenic localization in the tissues of the young adult Paragonimus westermani, immunogold labeling method was applied using serum immunoglobulins(IgG) of the dog which infected with...
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Serum IgE levels in rats infected with Paragonimus westermani

Shin MH, Ryu JS, Min DY

Paragonimus westermani is a common fluke in Korea. The present study aimed to determine serum total IgE and specific IgG levels in experimental paragonimiasis of rats. Each Wistar rat was...
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ELISA of rat sera infected with Paragonimus iloktsuenensis

Lim BK, Lee OR, Nam HS

Enzyme-linked immunosorbent assay (ELISA) of paragonimiasis iloktsuenensis rat sera was performed using crude antigens of Paragonimus iloktsuenensis (PIA), P. westermani (PWA) and Clonorchis sinensis (CSA). Three crude antigens (PIA, PWA,...
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Serodiagnosis of human paragonimiasis by ELISA-inhibition test using monoclonal antibodies

Yong TS, Seo JH, Yeo IS

ELISA-inhibition test using Paragonimus westermani specific monoclonal antibody (Mab) was investigated to improve the diagnostic specificity of paragonimiasis. By cell fusion, one hybridoma clone secreting anti-P. westermani specific Mab was...
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Immunohistochemical study on the antigenicity of body compartments of Paragonimus westermani

Lee SH, Sung SH, Chai JY

Production of circulating specific antibodies to the lung fluke (Paragonimus westermani) by its host is well known and used in various kinds of immunodiagnostic methods. However, it has not been...
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The effects of antibodies and complement in macrophage-mediated cytotoxicity on metacercariae of the lung fluke, Paragonimus westermani

Min DY, Ahn MH, Kim KM, Leem MH, Park SY

Paragonimus westermani is a tissue migrating parasite in the early stage until arriving at lung, and most of the parasites spend their life spans there. Considerable immune responses including activation...
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Changes of IgE production, splenic helper and suppressor T lymphocytes in mice infected with Paragonimus westermani

Min DY, Ryu JS, Shin MH

Effects of Paragonims westermani infection were observed in mice on the change of serum IgE level, the number of peripheral eosinophils and the distribution of Thy 1.2+ (CD3), L3T4+ (CD4),...
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Demonstration of species-specific and cross reactive components of Paragonimus westermani crude worm antigen by EITB

Joo KH, Ahn H, Chung MS, Rim HJ

Enzyme-linked immunoelectrotransfer blot (EITB) using crude worm antigen of adult Paragonimus westermani was performed for human patients sera to identify the species-specific components. Crude antigen was obtained by homogenizing and...
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Analysis of antigenic specificities of Paragonimus westermani developmental stages using immunoblot technique

Joo KH, Hong SC, Chung MS, Rim HJ

Serodiagnosis of parasitic infections is widely used, since parasites or their eggs are not always detected by ordinary methods. The sensitive tests such as ELISA are highly dependent on the...
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Antibody changes in paragonimiasis patients after praziquantel treatment as observed by ELISA and immunoblot

Cho SY, Kim SI, Kang SY, Kong Y, Han SK, Shim YS, Han YC

To observe antibody changes after praziquantel treatment in paragonimiasis, a total of 46 serum samples from 13 serologically diagnosed patients was collected for 4-28 months. The specific antibody (IgG) levels...
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