- HLA-DQA1 and DQB1 DNA typing by polymerase chain reaction using various allele-specific primers without sequence-specific oligonucleotide probes
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Han H, Rhyu MG, Kim TG, Kim SY, Chung YJ
- KMID: 1687935
- J Korean Soc Microbiol.
- 1991 Dec;26(6):585-593.
No abstract available. -
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- Detection of cytomegalovirus DNA in urine culture using polymerase chain reaction
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Sohn YM, Lee WY
- KMID: 1945940
- J Korean Pediatr Soc.
- 1993 May;36(5):626-633.
Polymerase chain reaction (PCR) amplication was used to detect cytomegalovirus (CMV) in tissue culture from the urine of newborns and patients who was suspected CMV infection, Synthetic oligonucleotide primer pairs... -
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- Detection of Mycobactrium tuberculosis by in situ hybridization
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Park CS, Lee JS, Jung JJ, Kim DH, Kim J
- KMID: 2160222
- Tuberc Respir Dis.
- 2000 May;48(5):699-708.
- doi: 10.4046/trd.2000.48.5.699
BACKGROUND: A presumptive histopathologic diagnosis of tuberculosis is commonly based on the finding of acid- fast bacilli upon microscopic examination of a diagnostic specimens. Although this traditional histochemical staining methodis... -
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- Comparison of Three Radiolabeled Probes for PCR-Hybridization to Detect Mycobacterium tuberculosis
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Jang SJ, Kang JS, Kim JH, Cho SS, Park G, Moon DS, Park YJ
- KMID: 1866517
- Korean J Lab Med.
- 2003 Feb;23(1):25-31.
BACKGROUND: Three homemade radiolabeled probes to detect DNA of Mycobacterium tuberculosis by PCR-hybridization (PCRH) assay were compared in order to select the most sensitive and economic probe with the longest... -
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- Analysis of the factors affecting the accuracy of detection for single base alterations by oligonucleotide microarray
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Liu S, Li Y, Fu X, Qiu M, Jiang B, Wu H, Li R, Mao Y, Xie Y
- KMID: 755744
- Exp Mol Med.
- 2005 Apr;37(2):71-77.
The oligonucleotide microarray, a high-throughput polymorphism detection technology, holds great promise for the characterization of complex genetic variance. To achieve greater sensitivity and specificity for it to be an effective... -
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- The Effects of Unilateral Dopaminergic Deafferentation on the Expression of mRNAs of Neurotrophic Factors
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Park MK, Kim H, Lee DH
- KMID: 1957217
- J Korean Neurol Assoc.
- 1995 Sep;13(3):425-437.
Recently protective and supportive functions of neurotrophic factors on dopaminergic neurons have been reported. In this study, in situ hybridization histochemistry with "S-labeled oligonucleotide probes for brain-derived factor (BDNF) and... -
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- Expression of EBNA-1 and EBNA-2 in Epstein-Barr Virus Associated Tumors
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Park CS, Choi HR, Kim HS, Kim DH, Kim J
- KMID: 2275008
- Chonnam Med J.
- 2001 Jun;37(2):149-157.
Expression of Epstein-Barr virus (EBV) genome in EBV associated tumors was examined by immuno-histochemistry (IHC) and in situ hybridization (ISH) with biotinylated synthetic oligonucleotide probes specific to EBV-specific nuclear antigen-1... -
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- Study on the Expression of Vasoactive intestinal polypeptide, Vasopressin and Oxytocin mRNAs in the Rat Brain using Double in situ Hybridization Technique
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Park KH, Park HS, Yoo YB, Lee WJ, Hwang DH, Lee BL, Cha CI, Cho SS, Baik SH
- KMID: 1530286
- Korean J Anat.
- 1998 Dec;31(6):807-815.
The technique of in situ hybridization using synthetic oligonucleotides labelled by non-radioactive method was developed to localize vasoactive intestinal polypeptide, arginine-vasopressin and oxytocin mRNAs in the rat brain. Also double... -
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- Detection of the c-m c Oncogene Amplification in Ovarian Carcinomas by Differential Polymerase Chain Reaction
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Lyu GS, Park CK, Lee CG, Cho YH, Hwang YY, Lee JD
- KMID: 1979417
- Korean J Pathol.
- 1997 Jul;31(7):644-654.
The amplification of c-myc oncogene was evaluated in 42 cases of ovarian carcinomas to correlate with clinical parameters. Using oligonucleotide primers, sequences from the c-myc exon-3 gene and from a... -
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- Changes of Neuropeptides in the Basal Ganglia of Hemiparkinsonian Rat Model
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Park KW, Lee DH, Park MK, Kim BJ, Ko SB, Suh YS
- KMID: 1957292
- J Korean Neurol Assoc.
- 1994 Dec;12(4):604-613.
We used in situ hybridization histochemistry with synthetic oligonucleotide probes to examined the effects of unilateral 6-hydroxydopamine lesions (which eliminated tyrosine hydroxylase mRNA containing cells in mesencephalon) on enkephalin, dynorphin,... -
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- Identification of mutagenic site of c-H-ras oncogene damaged by N-acetoxyacetylaminofluorene(AAAF)
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Oh SH, Kim KH, Lee SE, Yeh BI
- KMID: 1729587
- Yonsei Med J.
- 1991 Sep;32(3):207-214.
- doi: 10.3349/ymj.1991.32.3.207
A molecularly cloned human cellular H-ras (c-H-ras) oncogene(pbc N1 plasmid) was treated with N-acetoxyacetylaminofluorene (AAAF) in vitro and subcloned into E.coli. This was done to identify the mutational changes at... -
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- HLA-DQA1, HLA-DQB1, HLA-DRB1 in Korean Patients with Pulmonary Tuberculosis-by the polymerase chain reaction-sequence specific oligonucleotide probes (PCR-SSOP) method
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Kim YR, Kim HJ, Cho YJ
- KMID: 1550834
- Korean J Infect Dis.
- 1999 Oct;31(5):402-409.
BACKGROUND: Pulmonary tuberculosis is affected by environmental factors, such as hygiene, nutrition, and socioeconomic status. Recently it has also been shown to be correlated with specific HLA types in foreign... -
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- Genotypes of Circumsporozoite of Plasmodium vivax in Korea
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Choi JS, Lim CS, Lee KN
- KMID: 2300551
- Korean J Clin Pathol.
- 1998 Dec;18(4):671-677.
BACKGROUND: More than 200 million people suffer from malaria worldwide. In Korea this infectious disease had been on a decreasing trend since 1930s and was considered to be eradicated from... -
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- Cross-resistance Between Rifampicin and Rifabutin and Its Relationship with rpoB Gene Mutations in Clinically Isolated MDR-TB Strains
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Kim BJ, Oh SH, Cho EJ, Park SK
- KMID: 2318816
- Tuberc Respir Dis.
- 2006 Feb;60(2):171-179.
BACKGROUND: Despite the emerging danger of MDR-TB to human beings, there have only been a limited number of drugs developed to treat MDR-TB since 1970. This study investigated the cross-resistance... -
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- Expression of Neurotrophic Factors and Their Receptors mRNAs in the Rat Dorsal Root Ganglion Following Peripheral Nerve Injury
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Baek SY, Yang MS, Moon YS, Kim BS, Kim JB, Kim JJ
- KMID: 2224583
- Korean J Anat.
- 1999 Apr;32(2):221-229.
Degenerative and regnerative changes are occurred in the dorsal root ganglion (DRG) cells after the peripheral nerve injury. This experiment aimed to study the changes of neurotrophic factors and their... -
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- HLA-DQA1, HLA-DQB1, and HLA-DRB1 Allele Frequency in Korean Patients with Schizophrenia
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Shin KS, Lee MS, Kim YR, Kim YT, Cho YJ
- KMID: 2340476
- J Korean Neuropsychiatr Assoc.
- 2000 Jul;39(4):746-753.
OBJECTIVE: The aim of this study was to investigate the correlation between HLA-DQA1, HLA-DQB1 and HLA-DRB1 alleles and disease susceptibility in Korean schizophrenic patients. METHODS: HLA-DQA1, HLA-DQB1, and HLA-DRB1 allele typing... -
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- Rapid and easy detection of Helicobacter pylori by in situ hybridization
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Park CS, Kim J
- KMID: 1062191
- J Korean Med Sci.
- 1999 Feb;14(1):15-20.
- doi: 10.3346/jkms.1999.14.1.15
Various in situ hybridization (ISH) methods have been used to identify Helicobacter pylori, a causative organism responsible for chronic gastritis and peptic ulcer disease, but they were hard to perform... -
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- Molecular Cloning and Nucleotide Sequence of a Gene Encoding Alcohol Dehydrogenase of Helicobacter pylori
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Rhee KH, Lee WK, Cho MJ, Baik SC, Jeon YS, Choi YJ, Ryu BD, Song JY, Lee IG, Choi SH
- KMID: 2099281
- J Korean Soc Microbiol.
- 1998 Apr;33(2):129-137.
Partially purified H. pylori ADH was used to determine the amino acid sequence of ADH N- terminus. The sequence of the ADH N-terminus was determined as MRVQSKGF. The genomic library... -
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- A study of HLA-DQA genotyping of hair DNA using the PCR method
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You JH, Rhee KS, Park JW
- KMID: 2104098
- J Korean Pediatr Soc.
- 1993 Aug;36(8):1156-1164.
The characterization of genetic variation at the level of DNA has generated significant advances in gene mapping and disease diagnosis, and forensic identification of individuals. It is now possible to... -
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- Role of Genetic Polymorphisms of Cytochrome P4502E1 in Koreans with Alcoholic Liver Disease
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Lee CH, Bak YT, Kim JH, Byun KS, Kwon SY, Yeon JE
- KMID: 2084746
- Korean J Gastroenterol.
- 1997 May;29(5):645-650.
BACKGROUND/AIMS: There is a great deal of evidence for genetic predisposition to alcogolism, but comsiderably less is known regarding predisposition to alcoholic liver diseasc(ALD) . The aim of this stydy... -
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